1H, 13C, and 15N resonance assignments of reduced GrxS14 from Populus tremula × tremuloides

被引:0
作者
Lei Wang
Xiaobai Ren
Yifei Li
Nicolas Rouhier
Jean-Pierre Jacquot
Changwen Jin
Bin Xia
机构
[1] Peking University,Beijing Nuclear Magnetic Resonance Center
[2] Peking University,College of Chemistry and Molecular Engineering
[3] Peking University,College of Life Science
[4] Ecophysiologie et Ecologie Fonctionnelles Université Henri Poincaré,Unité Mixte de Recherches INRA UHP 1136, Interaction Arbres Microorganismes, IFR 110 Genomique
来源
Biomolecular NMR Assignments | 2011年 / 5卷
关键词
Glutaredoxin; NMR chemical shift assignments; Poplar;
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学科分类号
摘要
GrxS14 is a monothiol Glutaredoxin (Grx) from Populus tremula × tremuloides, which has a CGFS active site. GrxS14 is located in the chloroplasts and has been found to occur ether as an apo form or as a holo form with a [2Fe-2S] cluster. The holo form contains two monomers of apo GrxS14 bridged by the iron sulphur center, in the presence of two external glutathione molecules (Bandyopadhyay et al. 2008). The NMR assignments of the GrxS14 are essential for its solution structure determination.
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页码:121 / 124
页数:3
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