Occurrence of Deformed wing virus, Chronic bee paralysis virus and mtDNA variants in haplotype K of Varroa destructor mites in Syrian apiaries

被引：0

作者：

Toufic Elbeaino

Nouraldin Daher-Hjaij

Faiz Ismaeil

Jamal Mando

Bassem Solaiman Khaled

Raied Abou Kubaa

机构：

[1] CIHEAM - Istituto Agronomico Mediterraneo,General Commission for Scientific Agricultural Research (GCSAR)

[2] Administration of Plant Protection Research,undefined

[3] Damascus University,undefined

[4] Faculty of Agriculture,undefined

[5] Plant Protection Division,undefined

[6] Ministry of Agriculture and Agrarian Reform,undefined

[7] Department of Plant Protection,undefined

来源：

Experimental and Applied Acarology | 2016年 / 69卷

关键词：

Syria; Viruses; Sequencing; mtDNA;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A small-scale survey was conducted on 64 beehives located in four governorates of Syria in order to assess for the first time the presence of honeybee-infecting viruses and of Varroa destructor mites in the country. RT-PCR assays conducted on 192 honeybees (Apis mellifera L.) using virus-specific primers showed that Deformed wing virus (DWV) was present in 49 (25.5 %) of the tested samples and Chronic bee paralysis virus (CBPV) in 2 (1.04 %), whereas Acute bee paralysis virus, Sacbrood virus, Black queen cell virus and Kashmir bee virus were absent. Nucleotide sequences of PCR amplicons obtained from DWV and CBPV genomes shared 95–97 and 100 % identity with isolates reported in the GenBank, respectively. The phylogenetic tree grouped the Syrian DWV isolates in one cluster, distinct from all those of different origins reported in the database. Furthermore, 19 adult V. destructor females were genetically analyzed by amplifying and sequencing four fragments in cytochrome oxidase subunit 1 (cox1), ATP synthase 6 (atp6), cox3 and cytochrome b (cytb) mitochondrial DNA (mtDNA) genes. Sequences of concatenated V. destructor mtDNA genes (2696 bp) from Syria were similar to the Korean (K) haplotype and were found recurrently in all governorates. In addition, two genetic lineages of haplotype K with slight variations (0.2–0.3 %) were present only in Tartous and Al-Qunaitra governorates.

引用

页码：11 / 19

页数：8

共 57 条

[1]

Altschul SF(1990)Basic local alignment search tool J Mol Biol 215 403-410

[2]

Stephen F(1998)Two genetically distinct populations of J Apicult Res 37 69-78

[3]

Gish W(2000) with contrasting reproductive abilities on Exp Appl Acarol 24 165-189

[4]

Miller W(2001) (Acari: Varroidae) is more than one species Appl Environ Microbiol 67 2384-2387

[5]

Myers EW(2004)Detection of acute bee paralysis virus and black queen cell virus from honeybees by reverse transcriptase PCR Apidologie 35 441-448

[6]

Lipman DJ(2004)Molecular evidence for transmission of Kashmir bee virus in honeybee colonies by ectoparasitic mite, Am Bee J 144 557-559

[7]

Anderson DL(1989)Detection of deformed wing virus infection in honey bees, Cladistics 5 164-166

[8]

Fuchs S(2006) L., in the United States Uludag Bee J 6 68-73

[9]

Anderson DL(1988)PHYLIP—phylogeny inference package (version 3.2) Nucleic Acids Res 16 1829-1836

[10]

Trueman JWH(2010)Use of a new designed container for the detection of varroa mites in adult bees Apidologie 41 181-193

← 1 2 3 4 5 6 →