MALDI imaging mass spectrometry for direct tissue analysis: a new frontier for molecular histology

被引：0
作者：
Axel Walch
Sandra Rauser
Sören-Oliver Deininger
Heinz Höfler
机构：
[1] Helmholtz Zentrum München,Institute of Pathology
[2] Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH),Institute of Pathology
[3] Bruker Daltonik GmbH,undefined
[4] Technische Universität München,undefined
来源：
Histochemistry and Cell Biology | 2008年 / 130卷
关键词：
MALDI imaging mass spectrometry; Tissue; In situ-proteomics; Pathology;
D O I：
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学科分类号：
摘要：
Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a powerful tool for investigating the distribution of proteins and small molecules within biological systems through the in situ analysis of tissue sections. MALDI-IMS can determine the distribution of hundreds of unknown compounds in a single measurement and enables the acquisition of cellular expression profiles while maintaining the cellular and molecular integrity. In recent years, a great many advances in the practice of imaging mass spectrometry have taken place, making the technique more sensitive, robust, and ultimately useful. In this review, we focus on the current state of the art of MALDI-IMS, describe basic technological developments for MALDI-IMS of animal and human tissues, and discuss some recent applications in basic research and in clinical settings.
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  • [1] Aebersold R(2001)Mass spectrometry in proteomics Chem Rev 101 269-295
  • [2] Goodlett DR(2006)Automated acoustic matrix deposition for MALDI sample preparation Anal Chem 78 827-834
  • [3] Aerni HR(2005)Direct molecular imaging of Lymnaea stagnalis nervous tissue at subcellular spatial resolution by mass spectrometry Anal Chem 77 735-741
  • [4] Cornett DS(2006)Gold-enhanced biomolecular surface imaging of cells and tissue by SIMS and MALDI mass spectrometry Anal Chem 78 734-742
  • [5] Caprioli RM(2007)Imaging mass spectrometry at cellular length scales Nat Protoc 2 1185-1196
  • [6] Altelaar AF(2003)Laser capture microdissection MALDI for direct analysis of archival tissue J Proteome Res 2 95-98
  • [7] van Minnen J(2006)Bradykinin-related peptides from Phyllomedusa hypochondrialis Peptides 27 2137-2146
  • [8] Jiménez CR(2005)Tissue profiling by mass spectrometry: a review of methodology and applications Mol Cell Proteomics 4 394-401
  • [9] Heeren RM(2005)Deciphering protein molecular signatures in cancer tissues to aid in diagnosis, prognosis, and therapy Cancer Res 65 10642-10645
  • [10] Piersma SR(1997)Molecular imaging of biological samples: localization of peptides and proteins using MALDI-TOF MS Anal Chem 69 4751-4760
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