A Transgenic MMTV-Flippase Mouse Line for Molecular Engineering in Mammary Gland and Breast Cancer Mouse Models

被引：0
作者：
Fabiana Lüönd
Ruben Bill
Andrea Vettiger
Heide Oller
Pawel Pelczar
Gerhard Christofori
机构：
[1] University of Basel,Department of Biomedicine
[2] Biozentrum University of Basel,Focal Area Infection Biology
[3] University of Basel,Center for Transgenic Models
来源：
Journal of Mammary Gland Biology and Neoplasia | 2019年 / 24卷
关键词：
Breast cancer; Flippase; Lineage tracing; Mammary gland; MMTV promoter; Transgenic mice;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Genetically engineered mouse models have become an indispensable tool for breast cancer research. Combination of multiple site-specific recombination systems such as Cre/loxP and Flippase (Flp)/Frt allows for engineering of sophisticated, multi-layered conditional mouse models. Here, we report the generation and characterization of a novel transgenic mouse line expressing a mouse codon-optimized Flp under the control of the mouse mammary tumor virus (MMTV) promoter. These mice show robust Flp-mediated recombination in luminal mammary gland and breast cancer cells but no Flp activity in non-mammary tissues, with the exception of limited activity in salivary glands. These mice provide a unique tool for studying mammary gland biology and carcinogenesis in mice.
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页码：39 / 45
页数：6
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