Transformation and regeneration of garlic (Allium sativum L.) by Agrobacterium-mediated gene transfer

被引：0
|
作者：
T. Kondo
H. Hasegawa
M. Suzuki
机构：
[1] Aomori Green BioCenter,
[2] 221-10,undefined
[3] Yamaguchi,undefined
[4] Nogi,undefined
[5] Aomori,undefined
[6] 030-0142,undefined
[7] Japan e-mail: msuzuki@mm.neweb.ne.jp Fax: +81-177-281017,undefined
来源：
Plant Cell Reports | 2000年 / 19卷
关键词：
Key words Agrobacterium tumefaciens; Allium sativum; Garlic; Genetic transformation; Transgenic plant;
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摘要：
 By using highly regenerative calluses, we developed a stable transformation system in garlic (Allium sativum L.). The temperature and number of days of co-cultivation with Agrobacterium tumefaciens was shown to be an important factor in transient expression of the uid A gene. After a culture period of 5 months in selection medium containing hygromycin, 20 shoots were induced from ca. 1000 calluses, among which 15 plants expressed β-glucuronidase activity upon staining with X-Gluc. Shoots developed into transgenic garlic after 1 month. Integration of the uid A gene was confirmed by Southern blot analysis for genomic DNA of transgenic garlic plants.
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页码：989 / 993
页数：4
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