Biosynthesis, Characterization, and Antifungal Activity of the Silver Nanoparticles Against Pathogenic Candida species

被引：35
作者：
Khatoon N. [1 ,2 ]
Mishra A. [1 ]
Alam H. [2 ]
Manzoor N. [2 ,3 ]
Sardar M. [1 ]
机构：
[1] Enzyme Technology Lab, Department of Biosciences, Jamia Millia Islamia
[2] Medical Mycology Lab, Department of Biosciences, Jamia Millia Islamia
[3] College of Applied Medical Sciences, Taibah University, Al-Madinah Al-Munawara
来源：
BioNanoScience | 2015年 / 5卷 / 02期
关键词：
Antifungal; Biosynthesis; Candida spp; Electron microscopy; Silver nanoparticles; Tulsi;
D O I：
10.1007/s12668-015-0163-z
中图分类号：
学科分类号：
摘要：
Alcoholic extract of tulsi leaves was used as a reducing and stabilizing agent for the synthesis of silver nanoparticles (AgNPs). The use of plant extract for the synthesis of nanoparticles is cost-effective, single step, and an ecofriendly process. The biosynthesized AgNPs were characterized using UV-Vis, transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) analysis. The AgNPs formed show surface plasmon resonance at 430 nm. They have a smooth surface with an average diameter of 2–7 nm. Their crystalline nature was confirmed by the XRD. The antifungal activity of the AgNPs was evaluated for opportunistic human fungal pathogens Candida albicans, Candida glabrata, and Candida tropicalis. The antifungal effect was determined by minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC), and disk diffusion assay. Further, to assess the pathogenicity, proteinase and phospholipase assays were performed. TEM analysis of treated Candida cells reveals that the AgNPs may be exerting antifungal activity by disrupting the cell membrane structure and integrity. Cytotoxicity of AgNPs was checked by performing hemolytic assay against human erythrocytes. At MIC values, AgNPs caused only 5.6 % lysis in RBCs which was very low in comparison to conventional antifungal agents. Hence, biosynthesized AgNPs using plant extracts have immense antifungal potential and can be used in the management of fungal infections. Further studies have to be done to understand their mode of action. © 2015, Springer Science+Business Media New York.
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页码：65 / 74
页数：9
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