High-performance liquid chromatography–high-resolution time-of-flight mass spectrometry in the identification and determination of penicillin and tetracycline group antibiotics in food

A method of the identification and determination of 27 antibiotics of penicillin (15) and tetracycline (12) groups in food using high-resolution time-of-flight mass spectrometry combined with high-performance liquid chromatography and a simple and fast sample preparation procedure have been developed. The analytical range for penicillins is 3 (50)–300 (1000) μg/kg and for tetracyclines, 0.3 (1.5)–100 (200) μg/kg. A scheme of the identification and determination of antibiotics using the standard addition method has been proposed. The relative standard deviation of the results of analysis does not exceed 10%. The time of sample screening is 30–40 min, the time of the determination of the detected antibiotics is 2–3 h.