The role of anaerobic digestion in controlling the release of tetracycline resistance genes and class 1 integrons from municipal wastewater treatment plants

被引：0
作者：
Sudeshna Ghosh
Sara J. Ramsden
Timothy M. LaPara
机构：
[1] University of Minnesota,Department of Civil Engineering
[2] University of Michigan,Department of Civil and Environmental Engineering
[3] Barr Engineering,undefined
来源：
Applied Microbiology and Biotechnology | 2009年 / 84卷
关键词：
Anaerobic digestion; Antibiotic resistance; Municipal wastewater treatment; Tetracycline resistance;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In this study, the abilities of two anaerobic digestion processes used for sewage sludge stabilization were compared for their ability to reduce the quantities of three genes that encode resistance to tetracycline (tet(A), tet(O), and tet(X)) and one gene involved with integrons (intI1). A two-stage, thermophilic/mesophilic digestion process always resulted in significant decreases in the quantities of tet(X) and intI1, less frequently in decreases of tet(O), and no net decrease in tet(A). The thermophilic stage was primarily responsible for reducing the quantities of these genes, while the subsequent mesophilic stage sometimes caused a rebound in their quantities. In contrast, a conventional anaerobic digestion process rarely caused a significant decrease in the quantities of any of these genes, with significant increases occurring more frequently. Our results demonstrate that anaerobic thermophilic treatment was more efficient in reducing quantities of genes associated with the spread of antibiotic resistance compared to mesophilic digestion.
引用
收藏
页码：791 / 796
页数：5
相关论文
共 73 条
[1]  
Aitken MD(2005)Laboratory evaluation of thermophilic-anaerobic digestion to produce class A biosolids. 2. Inactivation of pathogens and indicator organisms in a continuous flow reactor followed by batch treatment Water Environ Res 77 3028-3036
[2]  
Sobsey MD(2005)Resistance to antibiotics: are we in the post-antibiotic era? Arch Med Res 36 697-705
[3]  
Shehee M(1995)Phylogenetic identification and in situ detection of individual microbial cells without cultivation Microbiol Rev 59 143-169
[4]  
Blauth KE(2002)Development, validation, and application of PCR primers for detection of tetracycline efflux genes of gram-negative bacteria Appl Environ Microbiol 68 1786-1793
[5]  
Hill VR(2007)Tetracycline resistance genes in activated sludge wastewater treatment plants Water Res 41 1143-1151
[6]  
Farrell JB(1980)Destruction by anaerobic mesophilic and thermophilic digestion of viruses and indicator bacteria indigenous to domestic sludges Appl Environ Microbiol 39 361-368
[7]  
Nappier SP(2001)Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance Microbiol Mol Biol Rev 65 232-260
[8]  
Walters GW(2004)Spatial variation in frequency and intensity of antibiotic interactions among Streptomycetes from prairie soil Appl Environ Microbiol 70 1051-1058
[9]  
Crunk PL(2007)Pandrug-resistant gram-bacteria in an urban wastewater treatment plant FEMS Microbiol Ecol 55 322-329
[10]  
van Abel N(2006)Persistence of Appl Environ Microbiol 72 565-574
12345678