Preparation and characterization of monoclonal antibodies to the cholera toxin

被引：0
|
作者：
E. E. Petrova
R. L. Komaleva
O. E. Lakhtina
L. V. Samokhvalova
N. A. Kalinina
N. S. Shoshina
A. Yu. Rubina
M. A. Filippova
Yu. V. Vertiev
T. I. Valyakina
Ye. V. Grishin
机构：
[1] Russian Academy of Sciences,Shemyakin
[2] Russian Academy of Sciences,Ovchinnikov Institute of Bioorganic Chemistry
[3] Russian Academy of Medical Sciences,Engelhardt Institute of Molecular Biology
来源：
Russian Journal of Bioorganic Chemistry | 2009年 / 35卷
关键词：
enzyme immunoassay; sandwich assay; biological microchip; B subunit of cholera toxin; cholera toxin; monoclonal antibodies;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Monoclonal antibodies to cholera toxin were obtained. They do not cross-react with the termolabile toxin (LT) of Escherichia coli, ricin, diphtherial toxin, staphylococcus enterotoxins of SEA, SEB, SEI, SEG, or the lethal factor and protective antigen of the anthrax toxin. Pairs of antibodies for the quantitative measurement of the cholera toxin in sandwich enzyme immunoassay (EIA) were selected. The detection limit of the toxin is 0.2 ng/ml for plate EIA and 0.44 ng/ml for microchip EIA. The presence of milk, broth, or surface water in the toxin samples does not reduce the sensitivity of EIA.
引用
收藏
页码：326 / 334
页数：8
相关论文
共 50 条
  • [1] Preparation and characterization of monoclonal antibodies to the cholera toxin
    Petrova, E. E.
    Komaleva, R. L.
    Lakhtina, O. E.
    Samokhvalova, L. V.
    Kalinina, N. A.
    Shoshina, N. S.
    Rubina, A. Yu.
    Filippova, M. A.
    Vertiev, Yu. V.
    Valyakina, T. I.
    Grishin, Ye. V.
    RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY, 2009, 35 (03) : 326 - 334
  • [2] Production and characteristics of monoclonal antibodies to the diphtheria toxin
    Valyakina, T. I.
    Lakhtina, O. E.
    Komaleva, R. L.
    Simonova, M. A.
    Samokhvalova, L. V.
    Shoshina, N. S.
    Kalinina, N. A.
    Rubina, A. Yu.
    Filippova, M. A.
    Vertiev, Yu. V.
    Grishin, E. V.
    RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY, 2009, 35 (05) : 556 - 565
  • [3] Production and characteristics of monoclonal antibodies to the diphtheria toxin
    T. I. Valyakina
    O. E. Lakhtina
    R. L. Komaleva
    M. A. Simonova
    L. V. Samokhvalova
    N. S. Shoshina
    N. A. Kalinina
    A. Yu. Rubina
    M. A. Filippova
    Yu. V. Vertiev
    E. V. Grishin
    Russian Journal of Bioorganic Chemistry, 2009, 35 : 556 - 565
  • [4] Generation and Characterization of Typhoid Toxin-Neutralizing Human Monoclonal Antibodies
    Jiao, Xuyao
    Smith, Sarah
    Stack, Gabrielle
    Liang, Qi
    Bradley, Allan
    Kellam, Paul
    Galan, Jorge E.
    INFECTION AND IMMUNITY, 2020, 88 (10)
  • [5] Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk
    He, Xiaohua
    McMahon, Stephanie
    Skinner, Craig
    Merrill, Paul
    Scotcher, Miles C.
    Stanker, Larry H.
    JOURNAL OF IMMUNOLOGICAL METHODS, 2013, 389 (1-2) : 18 - 28
  • [6] PREPARATION OF MONOCLONAL-ANTIBODIES TO STAPHYLOCOCCAL ALPHA-TOXIN BY INVITRO IMMUNIZATION
    BELYAEV, NN
    ZAKIRYANOVA, GK
    KOSTIN, GO
    TLEULIEVA, RT
    AGASHKIN, AO
    NAVASARDYANTS, DG
    EZEPCHUK, YV
    BEKLEMISHEV, AB
    BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 1991, 112 (07) : 999 - 1003
  • [7] PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO THE TOXIN COREGULATED PILUS (TCP) OF VIBRIO-CHOLERAE THAT PROTECT AGAINST EXPERIMENTAL CHOLERA IN INFANT MICE
    SUN, D
    TILLMAN, DM
    MARION, TN
    TAYLOR, RK
    SERODIAGNOSIS AND IMMUNOTHERAPY IN INFECTIOUS DISEASE, 1990, 4 (01) : 73 - 81
  • [8] Preparation and Characterization of The High Specificity Monoclonal Antibodies Against Citrinin
    Li Yong-Ning
    Wang Yuan-Yuan
    Zheng Yun-Quan
    Guo Yang-Hao
    PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 2010, 37 (11) : 1248 - 1253
  • [9] PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES WITH THERAPEUTIC POTENTIAL AGAINST SHIGA TOXIN
    ISLAM, MS
    STIMSON, WH
    JOURNAL OF CLINICAL & LABORATORY IMMUNOLOGY, 1990, 33 (01) : 11 - 16
  • [10] PREPARATION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO SEROTONIN BINDING-PROTEIN
    LIU, KP
    YU, PY
    HSIUNG, SH
    KIRCHGESSNER, AL
    GERSHON, MD
    TAMIR, H
    JOURNAL OF NEUROCHEMISTRY, 1990, 55 (03) : 1013 - 1021
12345