DDM1 (Decrease in DNA Methylation) genes in rice (Oryza sativa)

被引：0
作者：
Hiromi Higo
Muhammad Tahir
Kazuya Takashima
Asuka Miura
Koichi Watanabe
Akemi Tagiri
Masashi Ugaki
Ryuji Ishikawa
Mitsugu Eiguchi
Nori Kurata
Takuji Sasaki
Eric Richards
Makoto Takano
Naoki Kishimoto
Tetsuji Kakutani
Yoshiki Habu
机构：
[1] CREST,Department of Molecular Genetics
[2] Japan Science and Technology Agency,Department of Plant Science
[3] National Institute of Agrobiological Sciences,Department of Integrated Genetics
[4] University of Manitoba,Research Center for Advanced Science and Technology
[5] National Institute of Genetics,Graduate School of Frontier Sciences
[6] The University of Tokyo,Faculty of Agriculture and Life Science
[7] The University of Tokyo,Genetic Strains Research Center
[8] Hirosaki University,NODAI Research Institute
[9] Experimental Farm,Department of Biology
[10] National Institute of Genetics,Genetically Modified Organism Research Center
[11] National Institute of Genetics,Agrogenomics Research Center
[12] Tokyo University of Agriculture,undefined
[13] Washington University,undefined
[14] Boyce Thompson Institute for Plant Research,undefined
[15] National Institute of Agrobiological Sciences,undefined
[16] National Institute of Agrobiological Sciences,undefined
来源：
Molecular Genetics and Genomics | 2012年 / 287卷
关键词：
Rice; Arabidopsis; DNA methylation; Repeated sequences; Transposons;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Regulation of cytosine methylation in the plant genome is of pivotal in determining the epigenetic states of chromosome regions. Relative tolerance of plant to deficiency in cytosine methylation provides unparalleled opportunities to study the mechanism for regulation of cytosine methylation. The Decrease in DNA Methylation 1 (DDM1) of Arabidopsis thaliana is one of the best characterized plant epigenetic regulators that are necessary for maintenance of cytosine methylation in genomic DNA. Although cytosine methylation could affect various aspects of plant growth and development including those related to agricultural importance, orthologs of DDM1 in plants other than Arabidopsis has not been studied in detail. In this study, we identified two rice genes with similarity to Arabidopsis DDM1 and designated them OsDDM1a and OsDDM1b. Both of the rice DDM1 homologs are transcribed during development and their amino acid sequences are 93 % identical to each other. Transgenic rice lines expressing the OsDDM1a cDNA in the antisense orientation exhibited genomic DNA hypomethylation. In those lines, repeated sequences were more severely affected than a single copy sequence as is the case in Arabidopsis ddm1 mutants. Transcripts derived from endogenous transposon-related loci were up-regulated in the antisense OsDDM1 lines, opening a possibility to identify and utilize potentially active transposons for rice functional genomics.
引用
收藏
页码：785 / 792
页数：7
相关论文
共 195 条
[1]  
Brzeski J(2003)Deficient in DNA Methylation 1 (DDM1) defines a novel family of chromatin-remodeling factors J Biol Chem 278 823-828
[2]  
Jerzmanowski A(2002)Functional rice centromeres are marked by a satellite repeat and a centromere-specific retrotransposon Plant Cell 14 1691-1704
[3]  
Cheng Z(1984)Genomic sequencing Proc Natl Acad Sci USA 81 1991-1995
[4]  
Dong F(2001)Lsh, a member of the SNF2 family, is required for genome-wide methylation Genes Dev 15 2940-2944
[5]  
Langdon T(1996)Reduced DNA methylation in Proc Natl Acad Sci USA 93 8449-8454
[6]  
Ouyang S(2006) results in abnormal plant development EMBO Rep 7 1279-1284
[7]  
Buell CR(2001)Epigenetic regulation of transcription in intermediate heterochromatin Curr Opin Plant Biol 4 118-122
[8]  
Gu M(1992)Contribution of the Mol Gen Genet 233 209-216
[9]  
Blattner FR(1996) retrotransposon to rice functional genomics Proc Natl Acad Sci USA 93 7783-7788
[10]  
Jiang J(2000)Retrotransposon families in rice Plant Cell 12 357-369
12345678910