A novel human skin chamber model to study wound infection ex vivo

被引：0
作者：
Lars Steinstraesser
M. Sorkin
A. D. Niederbichler
M. Becerikli
J. Stupka
A. Daigeler
M. R. Kesting
I. Stricker
F. Jacobsen
M. Schulte
机构：
[1] Ruhr University School of Medicine,Department of Pathology
[2] Ruhr University Bochum,Department of Plastic Surgery, BG University Hospital Bergmannsheil
[3] Medizinische Hochschule Hannover,Department of Plastic, Hand and Reconstructive Surgery, Burn Center
[4] Technische Universität München,Department of Oral and Maxillofacial Surgery, Klinikum Rechts der Isar
来源：
Archives of Dermatological Research | 2010年 / 302卷
关键词：
Ex vivo; Infection; Human; Skin;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Wound infections with multi-drug resistant bacteria increase morbidity and mortality and have considerable socioeconomic impact. They can lead to impaired wound healing, resulting in rising treatment costs. The aim of this study was to investigate an ex vivo human wound infection model. Human full-thickness skin from the operating room (OR) was placed into the Bo-Drum® and cultivated for 7 days in an air–liquid interphase. On day 8, the skin was inoculated with either (1) Pseudomonas aeruginosa, (2) Staphylococcus aureus (105 CFU, n = 3) or (3) carrier control. 1, 3 and 7 days after inoculation colony forming units in the tissue/media were determined and cytokine expression was quantified. A reliable and reproducible wound infection could be established for 7 days. At this timepoint, 1.8 × 108 CFU/g tissue of P. aeruginosa and 2 × 107 CFU/g tissue of S. aureus were detected. Immunohistochemical analysis demonstrated bacterial infection and epidermolysis in infected skin. RT-PCR analysis exhibited a significant induction of proinflammatory cytokines after infection. The BO-drum® is a robust, easy-to-use, sterilizable and reusable ex vivo full-skin culture system. For investigation of wound infection, treatment and healing, the BO-drum® presents a convenient model and may help to standardize wound research.
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页码：357 / 365
页数：8
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