Hydrophobic interactions are the prevalent force in bromelain:Fab’ complex

被引：0
作者：
P. Gupta
M. Saleemuddin
R. H. Khan
机构：
[1] Aligarh Muslim University,Department of Biochemistry, Faculty of Life Sciences
[2] University of Minnesota,Department of Pharmacology, Basic Science Biomedical Engineering
[3] Aligarh Muslim University,Interdisciplinary Biotechnology Unit, Faculty of Life Sciences
来源：
Biochemistry (Moscow) | 2006年 / 71卷
关键词：
bromelain; Fab’; immunocomplexes; hydrophobic interaction; electrostatic interaction; methanol; NaCl;
D O I：
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中图分类号：
学科分类号：
摘要：
Antibromelain polyclonal antibodies against stem bromelain were raised in male albino rabbits and the Fab’ monomers isolated from the IgG of the immune sera as reported in our earlier communication (Gupta, P., Khan, R. H., and Saleemuddin, M. (2003) Biochim. Biophys. Acta, 1646, 131–135). Further, as evident from that communication, bromelain:Fab’ complex has 1: 1 stoichiometry. The stability of bromelain:Fab’ complex (1: 1) was investigated by far and near-UV CD and fluorescence measurements. Addition of up to 1.8 M NaCl caused no significant changes in fluorescence signals and near-UV CD peak pattern. However, the spectral studies together with gel filtration studies suggest dissociation of the complex beyond 5% (v/v) methanol. These results show that hydrophobic interactions play a pronounced role in the binding of Fab’ to bromelain while electrostatic interactions may be less crucial.
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页码：S31 / S37
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