Complete sequence of a viral nervous necrosis virus (NNV) isolated from red-spotted grouper (Epinephelus akaara) in China

被引：0
作者：
Hong Liu
Yong Teng
Xiaocong Zheng
Yurong Wu
Xiayang Xie
Junqiang He
Yiyou Ye
Zhixin Wu
机构：
[1] Shenzhen Exit & Entry Inspection and Quarantine Bureau,
[2] Medical College of Georgia,undefined
[3] College of Fisheries,undefined
[4] Huazhong Agricultural University,undefined
来源：
Archives of Virology | 2012年 / 157卷
关键词：
Coat Protein; Calculated Molecular Mass; Nervous Necrosis Virus; Striped Jack Nervous Necrosis Virus; Golden Pompano;
D O I：
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中图分类号：
学科分类号：
摘要：
A nodavirus isolated from red-spotted grouper (Epinephelus akaara) larvae in China has been subjected to genome analysis. The full-length genome sequences of RNA1 and RNA2 were determined, and the 5′-non-coding region (NCR) and 3′NCR sequences were determined by 5′ rapid amplification of cDNA ends (RACE) and 3′RACE. RNA1 is 3,103 nt in length and contains a 982-amino-acid open reading frame (ORF) encoding protein A with a calculated molecular mass of 110.74 kDa. RNA2 is 1,433 nt long and contains a 338-amino-acid major ORF encoding coat protein with a calculated molecular mass of 37.059 kDa. Multiple alignment and phylogenetic analysis clearly supported including this virus in the species Redspotted grouper nervous necrosis virus, genus Betanodavirus, family Nodaviridae.
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页码：777 / 782
页数：5
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