The Role of δ2-Opioid Receptors in the Regulation of Tolerance of Isolated Cardiomyocytes to Hypoxia and Reoxygenation

被引：0
作者：
A. V. Mukhomedzyanov
S. V. Popov
N. V. Naryzhnaya
V. N. Azev
L. N. Maslov
机构：
[1] Cardiology Research Institute,
[2] Tomsk National Research Medical Center,undefined
[3] Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences,undefined
来源：
Bulletin of Experimental Biology and Medicine | 2024年 / 176卷
关键词：
cardiomyocytes; hypoxia/reoxygenation; opioid receptors;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Hypoxia (20 min) and reoxygenation (30 min) were simulated on isolated rat cardiomyocytes to evaluate the cytoprotective effect of selective δ2-opioid receptor agonist deltorphin II, opioid receptor antagonist naloxone methiodide, μ-opioid receptor antagonist CTAP, κ-opioid receptor antagonist nor-binaltorphimine, ε1-opioid receptor antagonist BNTX, and δ2-opioid receptors naltriben. Deltorphin II was administered 5 min before reoxygenation, antagonists were administered 10 min before reoxygenation. The cytoprotective effect of deltorphin II was assessed by the number of cardiomyocytes survived after hypoxia/reoxygenation, as well as by the lactate dehydrogenase content in the incubation medium. It has been established that the cytoprotective effect of deltorphin II occurs at a concentration of 64 nmol/liter and is associated with activation of δ2-opioid receptors.
引用
收藏
页码：433 / 436
页数：3
相关论文
共 58 条
  • [1] Menees DS(2013)Door-to-balloon time and mortality among patients undergoing primary PCI N. Engl. J. Med. 369 901-909
  • [2] Peterson ED(2021)Use of intravascular imaging in patients with ST-segment elevation acute myocardial infarction Cardiovasc. Revasc. Med. 30 59-64
  • [3] Wang Y(2016)Prospects for creation of cardioprotective and antiarrhythmic drugs based on opioid receptor agonists Med. Res. Rev. 36 871-923
  • [4] Curtis JP(2009)Intrathecal morphine preconditioning induces cardioprotection via activation of delta, kappa, and mu opioid receptors in rats Anesth. Analg. 108 23-29
  • [5] Messenger JC(2020)Impact of morphine treatment on infarct size and reperfusion injury in acute reperfused ST-elevation myocardial infarction J. Clin. Med. 9 735-253
  • [6] Rumsfeld JS(2019)Participation of opioid receptors in the cytoprotective effect of chronic normobaric hypoxia Physiol. Res. 68 245-undefined
  • [7] Gurm HS(2021)Activation of peripheral δ2-opioid receptor prevents reperfusion heart injury Eur. J. Pharmacol. 907 undefined-undefined
  • [8] Megaly M(undefined)undefined undefined undefined undefined-undefined
  • [9] Pershad A(undefined)undefined undefined undefined undefined-undefined
  • [10] Glogoza M(undefined)undefined undefined undefined undefined-undefined
123456