Extraction of sage (Salvia officinalis L.) by pressurized hot water and conventional methods: antioxidant activity of the extracts

被引:0
|
作者
Maarit Ollanketo
Anna Peltoketo
Kari Hartonen
Raimo Hiltunen
Marja-Liisa Riekkola
机构
[1] Laboratory of Analytical Chemistry,
[2] Department of Chemistry,undefined
[3] P.O. Box 55,undefined
[4] FIN-00014 University of Helsinki,undefined
[5] Finland,undefined
[6] Division of Pharmacognosy,undefined
[7] Department of Pharmacy,undefined
[8] P.O. Box 56,undefined
[9] FIN-00014 University of Helsinki,undefined
[10] Finland,undefined
来源
European Food Research and Technology | 2002年 / 215卷
关键词
Sage Salvia officinalis L. Extraction techniques Pressurized hot water Phenolic antioxidants 1,1-Diphenyl-2-picrylhydrazyl radical Free radical scavenging activity;
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摘要
The effectiveness of the extraction of antioxidative compounds from sage (Salvia officinalis L.) by pressurized hot water extraction, ultrasonication-assisted methanol extraction, hydrodistillation, and maceration with 70% ethanol was evaluated by determining the capability of the extracts to scavenge the free radical DPPH· (1,1-diphenyl-2-picrylhydrazyl) in vitro. Pressurized hot water extraction was found to be the most effective extraction procedure, followed by maceration with 70% ethanol, hydrodistillation, and ultrasonication-assisted methanol extraction. In addition to the total extract, special attention was paid to rosmarinic and carnosic acids, carnosol and methyl carnosate. The extracts were analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC). The identification of compounds was confirmed by coupling RP-HPLC to mass spectrometry with electrospray ionization.
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页码:158 / 163
页数:5
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