In vitro culture, plant regeneration and clonal behaviour of Sesuvium portulacastrum (L.) L.: A prospective halophyte

被引：12
作者：
Lokhande V.H. [1 ]
Nikam T.D. [1 ]
Ghane S.G. [1 ]
Suprasanna P. [2 ]
机构：
[1] Department of Botany, University of Pune, Ganeshkhind
[2] Functional Plant Biology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay
来源：
Physiology and Molecular Biology of Plants | 2010年 / 16卷 / 2期
关键词：
clonal responses; regeneration; restoration; Sesuvium portulacastrum; tissue culture;
D O I：
10.1007/s12298-010-0020-z
中图分类号：
学科分类号：
摘要：
An efficient plant regeneration protocol using axillary shoots of the salt accumulator halophyte, Sesuvium portulacastrum (L.) L. was established and in vitro responses of six Sesuvium clones were studied. The shoot and root induction responses to cytokinins and auxins in clone MH (Maharashtra) were concentration specific. Significantly the highest number of shoots, average shoot elongation and percent shoot regeneration per explant were observed on MS medium supplemented with 40 μM 2-isopentenyl adenine (2iP) followed by 20 μM benzyladenine (BA). Higher cytokinin (60 μM), however, inhibited shoot induction and shoot length. The lower concentrations (5 or 10 μM) of α-napthaleneacetic acid (NAA) proved more effective for root induction, number of roots and average root length. Well-developed plantlets were successfully hardened and established in the field with more than 85 % survival rate. In vitro response of six Sesuvium clones cultured on MS + 20 μM BA revealed higher multiplication rate in clone MH and KA (Karnataka, India) compared to other clones. The results offer the prospect of selecting clones of this species with characteristics desirable for utilization and/or restoration in specific ecological zones. © 2010 Prof. H.S. Srivastava Foundation for Science and Society.
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页码：187 / 193
页数：6
相关论文
共 27 条
  • [1] Abdulaziz M.A.B., Jammel M.A.K., Micropropagation of grey mangrove Avicennia marina, Plant Cell Tiss. Org. Cult., 72, pp. 87-93, (2003)
  • [2] Amor N.B., Hamid K.B., Debez A., Grignon C., Abdelly C., Physiological and antioxidant responses of the perennial halophyte Crithmum maritimum to salinity, Plant Sci., 168, pp. 889-899, (2005)
  • [3] Balasubramanian R., Thilo R., Ahmed D., Ralf S., Bernhard H., Ahlert S., Jutta P., Aster tripolium L. and Sesuvium portulacstrum L.: two halophytes, two strategies to survive in saline habitats, Plant Physiol. Biochem., 44, pp. 395-408, (2006)
  • [4] Barrueto C.L.P., Machado A.C.M.G., Carvalheira S.B.R.C., Brasileiro A.C.M., Plant regeneration from seedling explants of Eucalyptus grandis x E. Urophylla, Plant Cell Tiss. Org. Cult., 56, pp. 17-22, (1999)
  • [5] Bhau B.S., Wakhlu A.K., Effect of genotype, explant type and growth regulators on organogenesis in Morus alba, Plant Cell Tiss. Org. Cult., 66, pp. 25-29, (2001)
  • [6] Cherian S., Reddy M.P., Evaluation of NaCl tolerance in the callus cultures of Suaeda nudiflora Moq, Biol. Plant., 46, pp. 193-198, (2003)
  • [7] Giridhar P., Gururaj H.B., Ravishankar G.A., In vitro shoot multiplication through shoot tip cultures of Decalepis hamiltonii Wight and Arn., A threatened plant endemic to Southern India, In Vitro Cell. Dev. Biol. Plant, 41, pp. 77-80, (2005)
  • [8] Grigoriadou K., Maloupa E., Micropropagation and salt tolerance of in vitro grown Crithmum maritimum L, Plant Cell Tiss. Org. Cult., 94, pp. 209-217, (2008)
  • [9] Jayanand B., Sydarsanam G., Sharma K., An efficient protocol for the regeneration of whole plants of chickpea (Cicer arietinum L.) by using axillary meristem explants derived from in vitro-germinated seedlings, In Vitro Cell. Dev. Biol. Plant, 39, pp. 171-179, (2003)
  • [10] Joshi P., Dhawan V., Axillary shoot multiplication of Swertia chirayita (Roxb. Ex Fleming) H. Karst., a critically endangered medicinal herb of temperate Himalayas, In Vitro Cell. Dev. Biol. Plant, 43, pp. 631-638, (2007)
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