Metal ion accessibility of histidine-modified superfolder green fluorescent protein expressed in Escherichia coli

被引：0

作者：

Natta Tansila

Kristian Becker

Chartchalerm Isarankura Na-Ayudhya

Virapong Prachayasittikul

Leif Bülow

机构：

[1] Mahidol University,Department of Clinical Microbiology, Faculty of Medical Technology

[2] Lund University,Department of Pure and Applied Biochemistry, Center for Chemistry and Chemical Engineering

来源：

Biotechnology Letters | 2008年 / 30卷

关键词：

Accessible surface area; Fluorescent quenching; Green fluorescent protein; Histidine; Metal binding;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Green fluorescent protein (GFP) is frequently utilized for metal ion detection and quantification. To improve the metal binding potential of GFP, three residues (N146, F165, and L201) were substituted to histidines. Each variant responded differently upon interaction with metal ions. More than 80% of N146H, having the most accessible surface area, could bind to immobilized metal ions. However, only F165H exhibited significant differences in quenching by soluble metal ions (22% fluorescence decrease) in comparison with the template protein (12%). These findings can be utilized for designing GFP variants for metal binding and sensor applications.

引用

页码：1391 / 1396

页数：5