Construction of bienzyme biosensors based on combination of the one-step electrodeposition and covalent-coupled sol-gel process

被引：0
作者：
Feng Li
Zhen Wang
Yan Feng
机构：
[1] Qingdao University of Science and Technology,Key Laboratory of Eco
来源：
Science in China Series B: Chemistry | 2009年 / 52卷
关键词：
chitosan; GPTMS; glucose; biosensor;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Horseradish peroxidase (HRP) and glucose oxidase (GOD) bienzyme biosensor was constructed by in-situ formation of the organic-inorganic biocomposite film based on the one-step electrodeposition and covalent-coupled sol-gel process. The electrodeposition was performed in the solution containing functional inorganic precursor possessing the epoxy groups, γ-glycidoxypropyltrimethoxysiloxane (GPTMS), a biopolymer chitosan (CS), HRP and GOD. The covalent-coupled sol-gel process was formed by self-hydrolysis and self-condensation of GPTMS, followed by in-situ covalent cross-linking of CS, HRP and GOD through covalent reaction between amino groups and epoxy groups. The developed bienzyme biosensor presented high stability in acidic solution owing to the covalent-coupled organic-inorganic hybridization. Compared with the non-hybrid HRP-GOD/CS/Au electrode, the bienzyme biosensor of HRP-GOD/GPTMS/CS/Au showed improved sensitivity and a wider linear range for the determination of glucose. The linear response of the developed HRP-GOD/GPTMS/CS/Au biosensor for the determination of glucose ranged from 1 to 351 μmol/L with a detection limit of 0.3 μmol/L.
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页码：2269 / 2274
页数：5
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