Identification of guitarfish species Rhinobatos percellens, R. horkelli, and Zapteryx brevirostris (Chondrichthyes) using mitochondrial genes and RFLP technique

被引：0
作者：
T. C. Mariguela
B. De-Franco
T. V. V. Almeida
F. F. Mendonça
O. B. F. Gadig
F. Foresti
C. Oliveira
机构：
[1] Universidade Estadual Paulista,Laboratório de Biologia e Genética de Peixes, Departamento de Morfologia, Instituto de Biociências
[2] UNESP – Universidade Estadual Paulista,Campus Litoral Paulista
来源：
Conservation Genetics Resources | 2009年 / 1卷
关键词：
Conservation genetics; Mitochondrial genes; Commercial species; Rays; Species identification;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The RFLP represents a fast and non-expensive tool to access species identification and can be used by fishery and law enforcement authorities to gather data from legal and illegal ray fisheries. In the present study, partial sequences of 16S and COI mitochondrial genes were submitted to RFLP analysis and the results showed that there is a high interspecific variability and no intraspecific polymorphism, making them an useful marker for guitarfish identification. All samples of Rhinobatidae: Rhinobatos percellens, R. horkelli, and Zapteryx brevirostris were positively identified. The main contribution of these forensic identification techniques is the possibility of evaluating population genetics of species status in order to improve conservation plans involving these species.
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页码：393 / 396
页数：3
相关论文
共 34 条
[1]  
Aljanabi SM(1997)Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques Nucleic Acids Res 25 4692-4693
[2]  
Martinez I(2005)Mitochondrial gene sequences useful for species identification of western North Atlantic Ocean sharks Fish Bull 103 516-523
[3]  
Greig TW(1999)BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT Nucleic Acids Symp Ser 41 95-98
[4]  
Moore KM(2001)Shark fishing in fin soup Conserv Genet 2 69-72
[5]  
Woodley CM(2009)Identification of shark and ray fins using DNA barcoding Fish Res 95 280-288
[6]  
Quattro JM(2009)Identification of the shark species Mol Ecol Resour 9 771-773
[7]  
Hall TA(2003)  Frente Marítimo 19 217-231
[8]  
Hoelzel AR(1998) and Mar Freshw Res 41 663-678
[9]  
Holmes BH(2000)  ICES J Mar Sci 57 476-494
[10]  
Steinke D(2003) (Elasmobranchii, Carcharhinidae) by multiplex PCR and PCR-RFLP techniques Nucleic Acids Res 31 3688-3691
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