Simultaneous LC-MS-MS Analysis of Valsartan and Hydrochlorothiazide in Human Plasma (vol 69, pg 1055, 2009)

被引：0

作者：

Shah, Hiten Janardan ^{[1
,2
]}

Kataria, Naresh B. ^{[1
]}

Subbaiah, Gunta ^{[1
]}

Patel, Chhagan N. ^{[1
]}

机构：

[1] Torrent Res Ctr, Bioanalyt Lab, Gandhinagar 382428, Gujarat, India

[2] Shri Sarvajanik Pharm Coll, Mehsana 384001, Gujarat, India

来源：

CHROMATOGRAPHIA | 2009年 / 69卷 / 9-10期

关键词：

Column liquid chromatography-tandem mass spectrometry; Selective reaction monitoring; Valsartan and hydrochlorothiazide;

D O I：

10.1365/s10337-009-1074-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and specific liquid chromatographic-tandem mass spectrometric method is described for simultaneous analysis of valsartan (VAL) and hydrochlorothiazide (HCTZ) in human plasma. VAL and HCTZ were chromatographed on a C-8 column with 75:15:10 (v/v) acetonitrile-methanol-0.001% aqueous ammonia as mobile phase. VAL and HCTZ were eluted at 0.69 min and 1.22 min, respectively, and, after electrospray ionization (ESI), detected in selected-reaction-monitoring mode. The precursor to product-ion transitions m/z 434.32 -> 179.22 and m/z 295.85 -> 204.86 were used to quantify VAL and HCTZ, respectively. Recovery by solid-phase extraction was > 90% for both analytes and the internal standard. The method was suitable for application to a pharmacokinetic study after oral administration of tablet containing 160 mg VAL and 25 mg HCTZ to 18 healthy volunteers.

引用

页码：1141 / 1141

页数：1