Single-molecule FRET of protein structure and dynamics - a primer

被引：0
作者：
Benjamin Schuler
机构：
[1] University of Zurich,Department of Biochemistry
来源：
Journal of Nanobiotechnology | / 11卷
关键词：
Single-molecule spectroscopy; fluorescence; Förster resonance energy transfer; correlation functions; fluorescence correlation spectroscopy; confocal detection; protein dynamics; protein folding;
D O I：
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学科分类号：
摘要：
Single-molecule spectroscopy has developed into a widely used method for probing the structure, dynamics, and mechanisms of biomolecular systems, especially in combination with Förster resonance energy transfer (FRET). In this introductory tutorial, essential concepts and methods will be outlined, from the FRET process and the basic considerations for sample preparation and instrumentation to some key elements of data analysis and photon statistics. Different approaches for obtaining dynamic information over a wide range of timescales will be explained and illustrated with examples, including the quantitative analysis of FRET efficiency histograms, correlation spectroscopy, fluorescence trajectories, and microfluidic mixing.
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  • [11] Hammes GG(2009)Direct observation of ultrafast folding and denatured state dynamics in single protein molecules Proc Natl Acad Sci USA 106 18569-18574
  • [12] Dittrich PS(2011)Fluorescence correlation spectroscopy of fast chain dynamics within denatured protein L Chemphyschem 12 696-703
  • [13] Muller B(2013)Measuring ultrafast protein folding rates from photon-by-photon analysis of single molecule fluorescence trajectories Chem Phys 422 229-237
  • [14] Schwille P(2002)Probing the free-energy surface for protein folding with single-molecule fluorescence spectroscopy Nature 419 743-747
  • [15] Henzler-Wildman KA(2011)Quantifying heterogeneity and conformational dynamics from single molecule FRET of diffusing molecules: recurrence analysis of single particles (RASP) Phys Chem Chem Phys 13 1857-1871
  • [16] Thai V(2011)Extracting Rate Coefficients from Single-Molecule Photon Trajectories and FRET Efficiency Histograms for a Fast-Folding Protein J Phys Chem A 115 3642-3656
  • [17] Lei M(2010)FRET efficiency distributions of multistate single molecules J Phys Chem B 114 15221-15226
  • [18] Ott M(2009)Experimental determination of upper bound for transition path times in protein folding from single-molecule photon-by-photon trajectories Proc Natl Acad Sci USA 106 11837-11844
  • [19] Wolf-Watz M(2012)Single-molecule fluorescence experiments determine protein folding transition path times Science 335 981-984
  • [20] Fenn T(2003)Watching proteins fold one molecule at a time Proc Natl Acad Sci USA 100 3197-3202
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