Single-molecule FRET of protein structure and dynamics - a primer

被引：0
作者：
Benjamin Schuler
机构：
[1] University of Zurich,Department of Biochemistry
来源：
Journal of Nanobiotechnology | / 11卷
关键词：
Single-molecule spectroscopy; fluorescence; Förster resonance energy transfer; correlation functions; fluorescence correlation spectroscopy; confocal detection; protein dynamics; protein folding;
D O I：
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学科分类号：
摘要：
Single-molecule spectroscopy has developed into a widely used method for probing the structure, dynamics, and mechanisms of biomolecular systems, especially in combination with Förster resonance energy transfer (FRET). In this introductory tutorial, essential concepts and methods will be outlined, from the FRET process and the basic considerations for sample preparation and instrumentation to some key elements of data analysis and photon statistics. Different approaches for obtaining dynamic information over a wide range of timescales will be explained and illustrated with examples, including the quantitative analysis of FRET efficiency histograms, correlation spectroscopy, fluorescence trajectories, and microfluidic mixing.
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