Identification of end-binding 1 (EB1) interacting proteins in Giardia lamblia

被引：0
作者：
Kyungpil Kang
Juri Kim
Tai-Soon Yong
Soon-Jung Park
机构：
[1] Yonsei University College of Medicine,Department of Environmental Medical Biology and Institute of Tropical Medicine, Post Brain Korea 21 Program
来源：
Parasitology Research | 2010年 / 106卷
关键词：
Indicator Plate; Candidate Protein; Putative Amino Acid Sequence; Adhesive Disk; Dropout Medium;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Yeast two-hybrid assays using the end-binding 1 (EB1) protein of Giardia lamblia as bait led to the isolation of four independent clones encoding γ-giardin, a neurogenic Notch-like protein, and two hypothetical proteins. Interactions between EB1 and the isolated proteins were confirmed by co-immunoprecipitation of in vitro-synthesized candidate proteins and EB1. Localization of these isolated proteins in G. lamblia was examined by immunofluorescence assays, indicating that they were predominantly present in cytoskeletal structures of G. lamblia. These results suggest that EB1 protein functions via interaction with the cytoskeletal components of G. lamblia.
引用
收藏
页码：723 / 728
页数：5
相关论文
共 48 条
  • [1] Bernander R(2001)Genomic ploidy in different stages of the Cell Microbiol 3 55-62
  • [2] Palm JE(2009) life cycle Cell Mol Life Sci 66 1631-1646
  • [3] Svard SG(1983)The Notch signaling pathway: transcriptional regulation at Notch target genes J Cell Sci 59 81-103
  • [4] Borggrefe T(2003)Characterization of proteins from the cytoskeleton of Int J Parasitol 33 3-28
  • [5] Oswald F(2003)The cytoskeleton of J Cell Biol 161 483-488
  • [6] Crossley R(1983)Spindle orientation in Trans R Soc Trop Med Hyg 77 487-488
  • [7] Holberton DV(2006) depends on the transport of microtubule ends along polarized actin cables Parasit Res 98 138-144
  • [8] Elmendorf HG(2008)Axenic culture of Parasitol Res 103 1459-1464
  • [9] Dawson SC(2008) in TYI-S-33 medium supplemented with bile Parasitol Int 57 465-471
  • [10] McCaffery JM(2000)Interaction of β-giardin with the Bop1 protein in Mol Biol Cell 11 2949-2959
12345