Sequence Analysis of the Nucleoprotein Gene of Asian Lineage Peste des Petits Ruminants Vaccine Virus

被引：0
作者：
D. Muthuchelvan
A. Sanyal
V. Balamurugan
P. Dhar
S. K. Bandyopadhyay
机构：
[1] Central Institute of Fisheries Technology,Division of Virology
[2] Project Directorate on Foot-and-Mouth Disease,undefined
[3] Indian Veterinary Research Institute,undefined
[4] Animal Husbandry Commissioner,undefined
来源：
Veterinary Research Communications | 2006年 / 30卷
关键词：
peste-des-petits ruminants virus; vaccine virus; N gene; nucleotide sequence; amino acid sequence;
D O I：
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摘要：
The complete nucleotide sequence of the nucleocapsid (N) protein of the peste-des-petits ruminants vaccine virus (PPRV Sungri/96) belonging to the Asian lineage was determined. The gene was 1692 nucleotides in length and encoded a polypeptide of 525 amino acids. The PPRV Sungri/96 N gene has a nucleotide homology of 92% for PPRV Nigeria 75/1 to 55.5% for canine distemper virus. At amino acid level the homology was 94.1% with PPRV Nigeria 75/1, while with other morbilliviruses, PPRV Sungri/96 had only 71.4–64.9% amino acid identity. The phosphorylation prediction reveals eight conserved sites across morbilliviruses, whereas in the C-terminal portion of the protein the sites are not conserved. Phylogenetic analysis of different N proteins of morbilliviruses revealed five well-defined clusters as observed previously. To the best of our knowledge this is the first report describing the nucleocapsid gene sequence of PPRV Indian isolate.
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页码：957 / 963
页数：6
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