Automated screening for small organic ligands using DNA-encoded chemical libraries

被引：0

作者：

Willy Decurtins

Moreno Wichert

Raphael M Franzini

Fabian Buller

Michael A Stravs

Yixin Zhang

Dario Neri

Jörg Scheuermann

机构：

[1] Institute of Pharmaceutical Sciences,Department of Chemistry and Applied Biosciences

[2] Swiss Federal Institute of Technology (ETH Zurich),undefined

[3] Present addresses: Roche Pharma Research and Early Development,undefined

[4] Roche Innovation Center,undefined

[5] Basel,undefined

[6] Switzerland (M.W.); Department of Medicinal Chemistry,undefined

[7] University of Utah,undefined

[8] Salt Lake City,undefined

[9] Utah,undefined

[10] USA (R.M.F.); Covagen AG,undefined

[11] Schlieren,undefined

[12] Switzerland (F.B.); Eawag,undefined

[13] Swiss Federal Institute of Aquatic Science and Technology,undefined

[14] Dübendorf,undefined

[15] Switzerland,undefined

[16] and Institute of Biogeochemistry and Pollutant Dynamics,undefined

[17] ETH Zürich,undefined

[18] Zürich,undefined

[19] Switzerland (M.A.S); B CUBE Center for Molecular Bioengineering,undefined

[20] Technische Universität Dresden,undefined

[21] Dresden,undefined

[22] Germany (Y.Z.).,undefined

来源：

Nature Protocols | 2016年 / 11卷

关键词：

D O I：

暂无

中图分类号：

学科分类号：

摘要：

This protocol describes an approach for screening DNA-encoded chemical libraries (DECLs) to identify molecules that bind to proteins of interest. After isolating binding library members, DNA barcodes are amplified and identified by high-throughput sequencing.

引用

页码：764 / 780

页数：16

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