Neurotoxicity evoked by N-methyl-d-aspartate in the organotypic static slice cultures of rat cerebral cortices: effect of GABAA receptor activation

被引：0

作者：

Makoto Yoshikawa

S. Goto

Akira Okamura

Tadashi Hamasaki

Yukitaka Ushio

机构：

[1] Laboratory for Cerebrovascular Disorders and Neuroscience,

[2] Department of Neurosurgery,undefined

[3] Kumamoto University Medical School,undefined

[4] Kumamoto 860,undefined

[5] Japan Tel.: 81-96-373-5220; Fax: 81-96-372-6140,undefined

来源：

Acta Neuropathologica | 1998年 / 95卷

关键词：

Key words Cerebral cortex; Organotypic slice culture; N-methyl-d-aspartate; Muscimol; Neuronal injury;

D O I：

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摘要：

We investigated the neurotoxicity evoked by N-methyl-d-aspartate (NMDA) receptor stimulation in the organotypic static slice cultures of rat cerebral cortices. We also examined whether the γ-aminobutyric acid (GABA)A receptor agonist muscimol has a protective effect on the NMDA-mediated neurotoxicity in this culture system. NMDA-mediated cytotoxicity was evaluated histologically and quantified by the measurement of lactate dehydrogenase (LDH) release into the culture medium. There was an NMDA-induced, dose-dependent leakage of LDH release and neuronal cell death, which were not attenuated by muscimol treatment. The results suggested that NMDA neurotoxicity is reproduced in the organotypic culture, and that GABAA receptor activation exerted no protective action against the NMDA cytotoxicity.

引用

页码：592 / 596

页数：4