Apoptotic Effect of Koumine on Human Breast Cancer Cells and the Mechanism Involved

被引：0
|
作者：
Xiaohua Zhang
Yi Chen
Bo Gao
Donglin Luo
Yayuan Wen
Xiaolin Ma
机构：
[1] Third Military Medical University,Department of Surgery for Breast and Thyroid, Institute of Surgery Research, Daping Hospital
[2] Third Military Medical University,Trauma Center, Research Institute of Surgery, Daping Hospital
来源：
Cell Biochemistry and Biophysics | 2015年 / 72卷
关键词：
Koumine; Breast cancer; Apoptosis; Cell cycle;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Koumine is an alkaloid separated from traditional Chinese herb Gelsemium elegans. In this study, anticancer activity and underlying mechanisms were investigated with an extract using human breast cancer cells. The survival rate was reduced in a concentration- and time-dependent manner as assessed by MTT assay. After incubation for 48 h, typical apoptotic morphological changes were observed by Hoechst 33258 dye assay. Flow cytometry result revealed that the treatment obviously induced G2/M arrest and apoptosis in MCF-7 cells. Furthermore, Western blotting demonstrated the down-regulation of protein expression of Bcl-2, whereas Bax and caspase-3 expressions were up-regulated. Therefore, we propose that koumine has the potential to be a future breast cancer chemotherapeutic agent.
引用
收藏
页码：411 / 416
页数：5
相关论文
共 50 条
  • [31] Number of apoptotic cells as a prognostic marker in invasive breast cancer
    De Jong J.S.
    Van Diest P.J.
    Baak J.P.A.
    British Journal of Cancer, 2000, 82 (2) : 368 - 373
  • [32] Number of apoptotic cells as a prognostic marker in invasive breast cancer
    de Jong, JS
    van Diest, PJ
    Baak, JPA
    BRITISH JOURNAL OF CANCER, 2000, 82 (02) : 368 - 373
  • [33] Molecular mechanism underlying the anticancer effect of simvastatin on MDA-MB-231 human breast cancer cells
    Shen, Yuan-Yuan
    Yuan, Yuan
    Du, Ying-Ying
    Pan, Yue-Yin
    MOLECULAR MEDICINE REPORTS, 2015, 12 (01) : 623 - 630
  • [34] An insight into the anticancer mechanism of Tribulus terrestris extracts on human breast cancer cells
    Patel, Apurva
    Soni, Anjali
    Siddiqi, Nikhat J.
    Sharma, Preeti
    3 BIOTECH, 2019, 9 (02)
  • [35] Riproximin modulates multiple signaling cascades leading to cytostatic and apoptotic effects in human breast cancer cells
    Pervaiz, Asim
    Zepp, Michael
    Adwan, Hassan
    Berger, Martin R.
    JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 2016, 142 (01) : 135 - 147
  • [36] Anticancer effects of purple carrot extract via induction of apoptotic genes on human breast cancer cells
    Park, Ha Young
    Lee, Sunhye
    Kim, So Hee
    Park, Jung Eun
    Hwang, Youn Seon
    Kang, Min Ho
    Chae, Seo Yeon
    Kim, Jin Woo
    FOOD SCIENCE AND BIOTECHNOLOGY, 2025, 34 (08) : 1737 - 1749
  • [37] An insight into the anticancer mechanism of Tribulus terrestris extracts on human breast cancer cells
    Apurva Patel
    Anjali Soni
    Nikhat J. Siddiqi
    Preeti Sharma
    3 Biotech, 2019, 9
  • [38] Riproximin modulates multiple signaling cascades leading to cytostatic and apoptotic effects in human breast cancer cells
    Asim Pervaiz
    Michael Zepp
    Hassan Adwan
    Martin R. Berger
    Journal of Cancer Research and Clinical Oncology, 2016, 142 : 135 - 147
  • [39] Counting of apoptotic cells: A methodological study in invasive breast cancer
    vandeSchepop, HAM
    deJong, JS
    vanDiest, PJ
    Baak, JPA
    JOURNAL OF CLINICAL PATHOLOGY-CLINICAL MOLECULAR PATHOLOGY EDITION, 1996, 49 (04): : M214 - M217
  • [40] Apoptotic mechanisms in T47D and MCF-7 human breast cancer cells
    L M Mooney
    K A Al-Sakkaf
    B L Brown
    P R M Dobson
    British Journal of Cancer, 2002, 87 : 909 - 917
12345