Holistic screening of collapsing honey bee colonies in Spain: A case study

被引：70

作者：

Cepero A. ^{[1
]}

Ravoet J. ^{[2
]}

Gómez-Moracho T. ^{[1
,3
,4
]}

Bernal J.L. ^{[5
]}

Del Nozal M.J. ^{[5
]}

Bartolomé C. ^{[3
,4
,6
]}

Maside X. ^{[3
,4
,6
]}

Meana A. ^{[7
]}

González-Porto A.V. ^{[8
]}

De Graaf D.C. ^{[2
]}

Martín-Hernández R. ^{[1
,9
]}

Higes M. ^{[1
]}

机构：

[1] Bee Pathology Laboratory, Consejería de Agricultura, Gobierno de Castilla-La Mancha, Centro Apícola Regional (CAR), Marchamalo

[2] Laboratory of Zoophysiology, Faculty of Sciences, Ghent University, Ghent

[3] Grupo de Medicina Xenómica, CIMUS, Universidade de Santiago de Compostela, Santiago De Compostela

[4] Xenómica Comparada de Parásitos Humanos, IDIS, Santiago De Compostela

[5] Analytical Chemistry Group, I.U.CINQUIMA, Universidad de Valladolid, Valladolid

[6] Departamento de Anatomía Patolóxica e Ciencias Forenses, Universidade de Santiago de Compostela, Santiago De Compostela

[7] Animal Health Department, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid

[8] Hive Products Laboratory, Consejería de Agricultura, Centro Apícola Regional, Marchamalo

[9] Instituto de Recursos Humanos Para la Ciencia y la Tecnología (INCRECYT-FEDER), Fundación Parque Científico y Tecnológico de Albacete, Albacete

来源：

BMC Research Notes | / 7卷 / 1期

关键词：

Colony collapse; Honeybee; Neonicotinoids; Palinology; Parasites; Viruses;

D O I：

10.1186/1756-0500-7-649

中图分类号：

学科分类号：

摘要：

Background: Here we present a holistic screening of collapsing colonies from three professional apiaries in Spain. Colonies with typical honey bee depopulation symptoms were selected for multiple possible factors to reveal the causes of collapse. Results: Omnipresent were Nosema ceranae and Lake Sinai Virus. Moderate prevalences were found for Black Queen Cell Virus and trypanosomatids, whereas Deformed Wing Virus, Aphid Lethal Paralysis Virus strain Brookings and neogregarines were rarely detected. Other viruses, Nosema apis, Acarapis woodi and Varroa destructor were not detected. Palinologic study of pollen demonstrated that all colonies were foraging on wild vegetation. Consequently, the pesticide residue analysis was negative for neonicotinoids. The genetic analysis of trypanosomatids GAPDH gene, showed that there is a large genetic distance between Crithidia mellificae ATCC30254, an authenticated cell strain since 1974, and the rest of the presumed C. mellificae sequences obtained in our study or published. This means that the latter group corresponds to a highly differentiated taxon that should be renamed accordingly. Conclusion: The results of this study demonstrate that the drivers of colony collapse may differ between geographic regions with different environmental conditions, or with different beekeeping and agricultural practices. The role of other pathogens in colony collapse has to bee studied in future, especially trypanosomatids and neogregarines. Beside their pathological effect on honey bees, classification and taxonomy of these protozoan parasites should also be clarified. © 2014Cepero et al.; licensee BioMed Central Ltd.

引用

共 66 条

[1]

Ball B.V., Allen M.F., The prevalence of pathogens in honey bee (apis mellifera) colonies infested with the parasitic mite varroa jacobsoni, Ann Appl Biol, 113, pp. 237-244, (1988)

[2]

Faucon J.P., Mathieu L., Ribiere M., Martel C.A., Drajnudel P., Zeggane S., Aurieres C., Aubert M.F.A., Honey bee winter mortality in France in 1999 and 2000, Bee World, 83, pp. 13-23, (2002)

[3]

Nordstrom S., Fries I., Aarhus A., Hansen H., Korpela S., Virus infections in nordic honey bee colonies with no, low or severe varroa jacobsoni infestations, Apidologie, 30, pp. 475-484, (1999)

[4]

Tentcheva D., Gauthier L., Zappulla N., Dainat B., Cousserans F., Colin M.E., Bergoin M., Prevalence and seasonal variations of six bee viruses in apis mellifera L. and varroa destructor mite populations in France, Appl Environ Microbiol, 70, pp. 7185-7191, (2004)

[5]

Cox-Foster D.L., Conlan S., Holmes E.C., Palacios G., Evans J.D., Moran N.A., Quan P.-L., Briese T., Hornig M., Geiser D.M., Martinson V., Vanengelsdorp D., Kalkstein A.L., Drysdale A., Hui J., Zhai J., Cui L., Hutchison S.K., Simons J.F., Egholm M., Pettis J.S., Lipkin W.I., A metagenomic survey of microbes in honey bee colony collapse disorder, Science, 318, pp. 283-287, (2007)

[6]

Higes M., Martin-Hernandez R., Meana A., Nosema ceranae, a new microsporidian parasite in honeybees in Europe, J Invertebr Pathol, 92, pp. 93-95, (2006)

[7]

Martin-Hernandez R., Meana A., Prieto L., Salvador A.M., Garrido-Bailon E., Higes M., Outcome of colonization of Apis mellifera by Nosema ceranae, Appl Environ Microbiol, 73, pp. 6331-6338, (2007)

[8]

Hatjina F., Tsoktouridis G., Bouga M., Charistos L., Evangelou V., Avtzis D., Meeus I., Brunain M., Smagghe G., De Graaf D.C., Polar tube protein gene diversity among Nosema ceranae strains derived from a Greek honey bee health study, J Invertebr Pathol, 108, pp. 131-134, (2011)

[9]

Soroker V., Hetzroni A., Yakobson B., David D., David A., Voet H., Slabezki Y., Efrat H., Levski S., Kamer Y., Klinberg E., Zioni N., Inbar S., Chejanovsky N., Evaluation of colony losses in Israel in relation to the incidence of pathogens and pests, Apidologie, 42, pp. 192-199, (2011)

[10]

Villa J.D., Bourgeois A.L., Danka R.G., Negative evidence for effects of genetic origin of bees on Nosema ceranae, positive evidence for effects of Nosema ceranae on bees, Apidologie, 44, pp. 511-518, (2013)

← 1 2 3 4 5 6 7 →