Force and compliance measurements on living cells using atomic force microscopy (AFM)

被引：97

作者：

Wojcikiewicz E.P. ^{[1
]}

Zhang X. ^{[1
]}

Moy V.T. ^{[1
]}

机构：

[1] Department of Physiology/Biophysics, Univ. of Miami School of Medicine, Miami, FL 33136

来源：

Biological Procedures Online | 2004年 / 6卷 / 1期

关键词：

Atomic force; Cell adhesion; Leukocytes; Microscopy;

D O I：

10.1251/bpo67

中图分类号：

学科分类号：

摘要：

We describe the use of atomic force microscopy (AFM) in studies of cell adhesion and cell compliance. Our studies use the interaction between leukocyte function associated antigen-1 (LFA-1)/intercellular adhesion molecule-1 (ICAM-1) as a model system. The forces required to unbind a single LFA-1/ICAM-1 bond were measured at different loading rates. This data was used to determine the dynamic strength of the LFA-1/ICAM-1 complex and characterize the activation potential that this complex overcomes during its breakage. Force measurements acquired at the multiple- bond level provided insight about the mechanism of cell adhesion. In addition, the AFM was used as a microindenter to determine the mechanical properties of cells. The applications of these methods are described using data from a previous study. © 2004. Biological Procedures Online.

引用

页码：1 / 9

页数：8

共 30 条

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