Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles

被引：0
作者：
Jesper Just
Yan Yan
Jean Farup
Peter Sieljacks
Mette Sloth
Morten Venø
Tingting Gu
Frank Vincenzo de Paoli
Jens Randel Nyengaard
Rikke Bæk
Malene Møller Jørgensen
Jørgen Kjems
Kristian Vissing
Kim Ryun Drasbek
机构：
[1] Center of Functionally Integrative Neuroscience,
[2] Dept of Clinical Medicine,undefined
[3] Aarhus University,undefined
[4] Interdisciplinary Nanoscience Center,undefined
[5] Aarhus University,undefined
[6] Dept of Molecular Biology and Genetics,undefined
[7] Aarhus University,undefined
[8] Section for Sport Science,undefined
[9] Department of Public Health,undefined
[10] Aarhus University,undefined
[11] Research laboratory for Biochemical Pathology,undefined
[12] Dept of Clinical Medicine,undefined
[13] Aarhus University,undefined
[14] Dept of Biomedicine,undefined
[15] Aarhus University,undefined
[16] Dept of Biomedicine – physiology,undefined
[17] Aarhus University,undefined
[18] Dept of Clinical Medicine,undefined
[19] Core Center for Molecular Morphology,undefined
[20] Section for Stereology and Microscopy,undefined
[21] Centre for Stochastic Geometry and Advanced Bioimaging,undefined
[22] Aarhus University,undefined
[23] Dept of Clinical Immunology,undefined
[24] Aalborg University Hospital,undefined
[25] Dept of Clinical Medicine,undefined
[26] Aalborg University,undefined
来源：
Scientific Reports | / 10卷
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摘要：
Ischemic exercise conducted as low-load blood flow restricted resistance exercise (BFRE) can lead to muscle remodelling and promote muscle growth, possibly through activation of muscle precursor cells. Cell activation can be triggered by blood borne extracellular vesicles (EVs) as these nano-sized particles are involved in long distance signalling. In this study, EVs isolated from plasma of healthy human subjects performing a single bout of BFRE were investigated for their change in EV surface profiles and miRNA cargos as well as their impact on skeletal muscle precursor cell proliferation. We found that after BFRE, five EV surface markers and 12 miRNAs were significantly altered. Furthermore, target prediction and functional enrichment analysis of the miRNAs revealed several target genes that are associated to biological pathways involved in skeletal muscle protein turnover. Interestingly, EVs from BFRE plasma increased the proliferation of muscle precursor cells. In addition, alterations in surface markers and miRNAs indicated that the combination of exercise and ischemic conditioning during BFRE can stimulate blood cells to release EVs. These results support that BFRE promotes EV release to engage in muscle remodelling and/or growth processes.
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