Characterization of the ZmDBP4 gene encoding a CRT/DRE-binding protein responsive to drought and cold stress in maize

被引：0
作者：
Chang-Tao Wang
Quan Yang
Yan-Mei Yang
机构：
[1] Beijing Technology and Business University,Beijing Key Lab of Plant Resources Research and Development
[2] Guangdong Pharmaceutical University,School of Traditional Chinese Medicine
[3] Hebei Polytechnic University,College of Science
来源：
Acta Physiologiae Plantarum | 2011年 / 33卷
关键词：
CBF/DREB; Abiotic stress; Induction kinetics; Cold tolerance; Transgenic plants; Maize;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
C-repeat/dehydration-responsive element binding factors (CBF/DREBs) play a vital regulatory role in abiotic stress responses in plants. We isolated the ZmDBP4 gene, a member of the A-1 subgroup of the CBF/DREB subfamily, from maize seedlings. A ZmDBP4::hGFP fusion protein was found to localize into the nucleus upon introduction into epidermal onion cells. As a trans-acting factor, the ZmDBP4 protein activated CRT/DRE-containing genes under normal growth conditions in transgenic Arabidopsis plants. ZmDBP4 transcription was highly activated by cold and moderately by drought in ABA-independent manner. Isolation of the ZmDBP4 promoter revealed some cis-acting elements responding to stresses. Overexpression of ZmDBP4 improved drought and cold-stress tolerance in transgenic Arabidopsis plants. Our results suggested that ZmDBP4 produces a functional factor that may play a regulatory role in abiotic stress response.
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页码：575 / 583
页数：8
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