An Organotypic Culture of the Newt Retina together with Other Tissues of the Posterior Eye Segment as a Model for Studying the Effects of Cell Adhesion Glycoproteins

被引：0
作者：
M. S. Krasnov
E. N. Grigoryan
V. P. Yamskova
机构：
[1] Kol'tsov Institute of Developmental Biology,Russian Academy of Sciences
来源：
Biology Bulletin of the Russian Academy of Sciences | 2003年 / 30卷
关键词：
Retina; Acetylcholinesterase; Pigment Epithelium; Organotypic Culture; Acetylcholinesterase Activity;
D O I：
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学科分类号：
摘要：
The adult newt retina explanted together with the posterior eye wall and cultivated for a short time in a serum-free medium was tested as an experimental model by several criteria, including the expression of protein markers of the main retinal cell types. Some differences in the expression of specific photoreceptor, interneuron, and glial cell proteins as well as the localization of acetylcholinesterase activity were found during in vitro cultivation. Using this model, preliminary tests of new cell adhesion glycoproteins from the bovine retina and pigment epithelium were conducted, and the role of pigment epithelial cell proteins in improving cell viability in the cultivated newt retina was revealed. Moreover, the fraction of basic adhesion proteins from the bovine pigment epithelium improved the survival potential of the macroglial (Muller) cell population, compared to that in the control.
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页码：17 / 29
页数：12
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