Thiamin biosynthesis in prokaryotes

被引：0

作者：

T. P. Begley

Diana M. Downs

Steven E. Ealick

Fred W. McLafferty

Adolphus P. G. M. Van Loon

Sean Taylor

Nino Campobasso

Hsiu-Ju Chiu

Cynthia Kinsland

Jason J. Reddick

Jun Xi

机构：

[1] Department of Chemistry and Chemical Biology,

[2] 120 Baker Laboratory,undefined

[3] Cornell University,undefined

[4] Ithaca,undefined

[5] NY 14853,undefined

[6] USA e-mail: tpb2@cornell.edu Tel. +1-607-255-7133; Fax +1-607-255-4137,undefined

[7] Department of Bacteriology,undefined

[8] University of Wisconsin-Madison,undefined

[9] 1550 Linden Drive,undefined

[10] Madison,undefined

[11] WI 53706,undefined

[12] USA,undefined

[13] Biotechnology Section,undefined

[14] F. Hoffmann-La Roche Ltd.,undefined

[15] Grenzacherstrasse 124,undefined

[16] CH-4070 Basel,undefined

[17] Switzerland,undefined

来源：

Archives of Microbiology | 1999年 / 171卷

关键词：

Key words Thiamin; Biosynthesis; Prokaryotes; Transport; Kinase; Mechanism; Structure;

D O I：

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中图分类号：

学科分类号：

摘要：

Twelve genes involved in thiamin biosynthesis in prokaryotes have been identified and overexpressed. Of these, six are required for the thiazole biosynthesis (thiFSGH, thiI, and dxs), one is involved in the pyrimidine biosynthesis (thiC), one is required for the linking of the thiazole and the pyrimidine (thiE), and four are kinase genes (thiD, thiM, thiL, and pdxK). The specific reactions catalyzed by ThiEF, Dxs, ThiDM, ThiL, and PdxK have been reconstituted in vitro and ThiS thiocarboxylate has been identified as the sulfur source. The X-ray structures of thiamin phosphate synthase and 5-hydroxyethyl-4-methylthiazole kinase have been completed. The genes coding for the thiamin transport system (thiBPQ) have also been identified. Remaining problems include the cloning and characterization of thiK (thiamin kinase) and the gene(s) involved in the regulation of thiamin biosynthesis. The specific reactions catalyzed by ThiC (pyrimidine formation), and ThiGH and ThiI (thiazole formation) have not yet been identified.

引用

页码：293 / 300

页数：7