A nanocomposite prepared from copper(II) and nitrogen-doped graphene quantum dots with peroxidase mimicking properties for chemiluminescent determination of uric acid

被引：0
作者：
Bingfang Shi
Yubin Su
Yan Duan
Shengyu Chen
Weiyuan Zuo
机构：
[1] Baise University,Key Laboratory of Regional Ecological Environment Analysis and Pollution Control of West Guangxi, College of Chemistry and Environmental Engineering
[2] Guangxi Normal University,State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences
来源：
Microchimica Acta | 2019年 / 186卷
关键词：
Chemiluminescence; Nanocomposite; Molecule detection; Urine;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Nitrogen-doped graphene quantum dots (N-GQD) were employed along with Cu(II) ions under alkaline conditions and room temperature to synthesize nanocomposites of type Cu(II)/Cu2O/N-GQDs. These nanocomposites exhibit excellent stability and dispersity, and also display a peroxidase-like activity that is superior to pure Cu2O nanoparticles and natural peroxidase (POx). A chemiluminescence (CL) method was designed that is based on the use of uricase which oxidizes uric acid under formation of H2O2. The nanocomposites were used as a POx mimic in the luminol-H2O2 CL system. Under optimized conditions, a linear relationship between CL intensity and the uric acid (UA) concentration in the range of 0.16—4.0 μM, and a detection limit of 0.041 μM (at S/N = 3) were obtained. The CL method was applied to the determination of UA in spiked serum and urine, and recoveries ranged from 85.0 to 121.3%.
引用
收藏
相关论文
共 166 条
  • [1] Kaur H(1990)Action of biologically-relevant oxidizing species upon uric acid. Identification of uric acid oxidation products Chem Biol Interact 73 235-247
  • [2] Halliwell B(2011)Electrochemical detection of uric acid in mixed and clinical samples: a review Electroanalysis 23 305-320
  • [3] Lakshmi D(2016)Development of a paper-based analytical device for colorimetric detection of uric acid using gold nanoparticles- graphene oxide (AuNPs-GO) conjugate Anal Methods 8 6965-6973
  • [4] Whitcombe MJ(2012)Quasi-real time quantification of uric acid in urine using boron doped diamond microelectrode with in situ cleaning Anal Chem 84 10207-10213
  • [5] Davis FP(2017)Gold nanoclusters as switch-off fluorescent probe for detection of uric acid based on the inner filter effect of hydrogen peroxide-mediated enlargement of gold nanoparticles Biosens Bioelectron 91 734-740
  • [6] Sharma S(2011)Point of care diagnostics: status and future Anal Chem 84 487-515
  • [7] Prasad BB(2016)Rapid and highly-sensitive uric acid sensing based on enzymatic catalysis-induced upconversion inner filter effect Biosens Bioelectron 86 109-114
  • [8] Kumar S(2015)Preparation of poly (3, 4-ethylenedioxythiophene) nanofibers modified pencil graphite electrode and investigation of over-oxidation conditions for the selective and sensitive determination of uric acid in body fluids Anal Chim Acta 891 312-320
  • [9] Bhushan P(2014)Flow injection chemiluminescence determination of naphazoline hydrochloride in pharmaceuticals Luminescence 29 48-51
  • [10] Bhattacharya S(2015)Cupric oxide nanoparticles-enhanced chemiluminescence method for measurement of β-lactam antibiotics Luminescence. 30 625-630
12345678910