Republished study: long-term toxicity of a Roundup herbicide and a Roundup-tolerant genetically modified maize

被引：128
作者：
Séralini G.-E. [1 ,3 ]
Clair E. [1 ,3 ]
Mesnage R. [1 ,3 ]
Gress S. [1 ,3 ]
Defarge N. [1 ,3 ]
Malatesta M. [2 ]
Hennequin D. [3 ]
de Vendômois J.S. [1 ,3 ]
机构：
[1] Institute of Biology, EA 2608 and CRIIGEN and Risk Pole, MRSH-CNRS, Esplanade de la Paix, University of Caen, Caen, 14032, Cedex
[2] Department of Neurological, Neuropsychological, Morphological and Motor Sciences, University of Verona, Verona
[3] Risk Pole, MRSH-CNRS, Esplanade de la Paix, University of Caen, Caen, 14032, Cedex
来源：
Environmental Sciences Europe | 2014年 / 26卷 / 01期
关键词：
Endocrine disruption; Genetically modified; Glyphosate-based herbicides; GMO; NK603; Rat; Roundup;
D O I：
10.1186/s12302-014-0014-5
中图分类号：
学科分类号：
摘要：
Background: The health effects of a Roundup-tolerant NK603 genetically modified (GM) maize (from 11% in the diet), cultivated with or without Roundup application and Roundup alone (from 0.1 ppb of the full pesticide containing glyphosate and adjuvants) in drinking water, were evaluated for 2 years in rats. This study constitutes a follow-up investigation of a 90-day feeding study conducted by Monsanto in order to obtain commercial release of this GMO, employing the same rat strain and analyzing biochemical parameters on the same number of animals per group as our investigation. Our research represents the first chronic study on these substances, in which all observations including tumors are reported chronologically. Thus, it was not designed as a carcinogenicity study. We report the major findings with 34 organs observed and 56 parameters analyzed at 11 time points for most organs. Results: Biochemical analyses confirmed very significant chronic kidney deficiencies, for all treatments and both sexes; 76% of the altered parameters were kidney-related. In treated males, liver congestions and necrosis were 2.5 to 5.5 times higher. Marked and severe nephropathies were also generally 1.3 to 2.3 times greater. In females, all treatment groups showed a two- to threefold increase in mortality, and deaths were earlier. This difference was also evident in three male groups fed with GM maize. All results were hormone- and sex-dependent, and the pathological profiles were comparable. Females developed large mammary tumors more frequently and before controls; the pituitary was the second most disabled organ; the sex hormonal balance was modified by consumption of GM maize and Roundup treatments. Males presented up to four times more large palpable tumors starting 600 days earlier than in the control group, in which only one tumor was noted. These results may be explained by not only the non-linear endocrine-disrupting effects of Roundup but also by the overexpression of the EPSPS transgene or other mutational effects in the GM maize and their metabolic consequences. Conclusion: Our findings imply that long-term (2 year) feeding trials need to be conducted to thoroughly evaluate the safety of GM foods and pesticides in their full commercial formulations. © 2014, Séralini et al.; licensee Springer.
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页码：1 / 17
页数：16
相关论文
共 73 条
[1]  
Seralini G.-E., Mesnage R., Clair E., Gress S., de Vendomois J., Cellier D., Genetically modified crops safety assessments: present limits and possible improvements, Environ Sci Eur, 23, (2011)
[2]  
Domingo J.L., Gine Bordonaba J., A literature review on the safety assessment of genetically modified plants, Environ Int, 37, pp. 734-742, (2011)
[3]  
Hammond B., Dudek R., Lemen J., Nemeth M., Results of a 13 week safety assurance study with rats fed grain from glyphosate tolerant corn, Food Chem Toxicol, 42, pp. 1003-1014, (2004)
[4]  
Hammond B., Lemen J., Dudek R., Ward D., Jiang C., Nemeth M., Burns J., Results of a 90-day safety assurance study with rats fed grain from corn rootworm-protected corn, Food Chem Toxicol, 44, pp. 147-160, (2006)
[5]  
Hammond B.G., Dudek R., Lemen J.K., Nemeth M.A., Results of a 90-day safety assurance study with rats fed grain from corn borer-protected corn, Food Chem Toxicol, 44, pp. 1092-1099, (2006)
[6]  
Seralini G.E., Cellier D., de Vendomois J.S., New analysis of a rat feeding study with a genetically modified maize reveals signs of hepatorenal toxicity, Arch Environ Contam Toxicol, 52, pp. 596-602, (2007)
[7]  
Spiroux de Vendomois J., Roullier F., Cellier D., Seralini G.E., A comparison of the effects of three GM corn varieties on mammalian health, Int J Biol Sci, 5, pp. 706-726, (2009)
[8]  
Gasnier C., Dumont C., Benachour N., Clair E., Chagnon M.C., Seralini G.E., Glyphosate-based herbicides are toxic and endocrine disruptors in human cell lines, Toxicology, 262, pp. 184-191, (2009)
[9]  
Benachour N., Seralini G.E., Glyphosate formulations induce apoptosis and necrosis in human umbilical, embryonic, and placental cells, Chem Res Toxicol, 22, pp. 97-105, (2009)
[10]  
Romano M.A., Romano R.M., Santos L.D., Wisniewski P., Campos D.A., de Souza P.B., Viau P., Bernardi M.M., Nunes M.T., de Oliveira C.A., Glyphosate impairs male offspring reproductive development by disrupting gonadotropin expression, Arch Toxicol, 86, pp. 663-673, (2012)
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