Expression of the renin angiotensin system genes in the kidney and heart of ISIAH hypertensive rats

被引：4
作者：
Fedoseeva L.A. [1 ]
Ryazanova M.A. [1 ]
Antonov E.V. [1 ]
Dymshits G.M. [1 ,2 ]
Markel A.L. [1 ,2 ]
机构：
[1] Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090
[2] Novosibirsk State University, Novosibirsk
来源：
Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry | 2011年 / 5卷 / 1期
基金：
俄罗斯基础研究基金会;
关键词：
arterial hypertension; ISIAH rat strain; real-time PCR; renin-angiotensin system;
D O I：
10.1134/S1990750811010069
中图分类号：
学科分类号：
摘要：
The content of mRNA of renin-angiotensin system (RAS) genes was measured in the kidney and heart of hypertensive ISIAH and normotensive WAG rats using the real-time PCR. A statistically significant decrease in the mRNA level of RAS genes was registered in the kidney of ISIAH rats, including Ren (by 45%), Ace (43%), AT1A (34%), COX-2 (50%). The level of myocardial expression of AT1A decreased by 28% while Ace expression increased by 80%. These results suggest reduction of renal RAS basal activity in the hypertensive ISIAH rats, and therefore this strain of rats may be referred to the group of models of low-renin hypertension. The ISIAH rats were also characterized by a two-fold increase in the connective tissue sodium concentration and also by a small (but statistically significant) increase in plasma sodium concentration (139 ± 0.3 mmol/l versus 136 ± 0.25 mmol/l in WAG rats). These results together with a tendency to a decrease of plasma aldosterone level also support existence of a classical low-renin hypertension in the ISIAH rats. It is suggested that altered function of renal ion channels represents a basis for the development of low renin hypertension in the ISIAH rats. In addition, impairments in renal system of NO synthesis may also contribute to the pathogenesis of arterial hypertension in the ISIAH rats. © 2011 Pleiades Publishing, Ltd.
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页码：37 / 43
页数：6
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