IP-10, MCP-1, MCP-2, MCP-3, and IL-1RA hold promise as biomarkers for infection with M. tuberculosis in a whole blood based T-cell assay

被引：103
作者：
Ruhwald M. [1 ,2 ]
Bjerregaard-Andersen M. [3 ,4 ]
Rabna P. [4 ]
Eugen-Olsen J. [2 ]
Ravn P. [2 ,5 ]
机构：
[1] Dep. of Infectious Diseases 144, Copenhagen University, Hvidovre Hospital
[2] Clinical Research Centre 136, Copenhagen University, Hvidovre Hospital
[3] Dep. of Infectious Diseases Q, Skejby University Hospital
[4] Bandim Health Project, 1004 Bissau Codex
[5] Department of Medicine, Copenhagen University, Herlev Hospital
来源：
BMC Research Notes | / 2卷 / 1期
关键词：
Tuberculosis; Tuberculin Skin Test; Diagnostic Potential; Interferon Gamma Release Assay; Sputum Smear Microscopy;
D O I：
10.1186/1756-0500-2-19
中图分类号：
学科分类号：
摘要：
Background. IFN- responses to M. tuberculosis antigens are used as in-vitro diagnostic tests for tuberculosis infection. The tests are highly specific but sensitivity may be impaired due to immuno-suppression. The objective of this small exploratory study was to compare three novel biomarkers for in-vitro diagnosis of tuberculosis - MCP-1, MCP-3 and IL-1RA - with the current established biomarker IFN- and the newly described IP-10 and MCP-2. Methods. Whole blood from 8 patents with active tuberculosis and from 7 healthy controls was stimulated with M. tuberculosis specific antigens and mitogen in the Quantiferon In Tube test tubes. Levels of biomarkers were measured using Luminex and ELISA (IFN-). Results. We found all five new biomarkers were expressed in significantly higher concentrations compared to IFN-. IP-10 and MCP-3 levels in the un-stimulated samples were higher in patients compared with controls. Conclusion. All biomarkers had diagnostic potential as they could differentiate between the patients and the controls. IP-10 and MCP-2 seemed most promising as they were expressed in high levels with antigen stimulation and were low in the un-stimulated samples. Further studies are needed to explore the potential of these highly expressed novel biomarkers individually and in combination. © 2009 Ruhwald et al.
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