Biological applications of microchip electrophoresis with amperometric detection: in vivo monitoring and cell analysis

被引：0
|
作者：
Kelci M. Schilly
Shamal M. Gunawardhana
Manjula B. Wijesinghe
Susan M. Lunte
机构：
[1] University of Kansas,Department of Chemistry
[2] University of Kansas,Ralph N. Adams Institute for Bioanalytical Chemistry
[3] University of Kansas,Department of Pharmaceutical Chemistry
来源：
Analytical and Bioanalytical Chemistry | 2020年 / 412卷
关键词：
Electrophoresis; Electrochemical detection; Microdialysis; Nitric oxide; Neurotransmitters;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Microchip electrophoresis with amperometric detection (ME-EC) is a useful tool for the determination of redox active compounds in complex biological samples. In this review, a brief background on the principles of ME-EC is provided, including substrate types, electrode materials, and electrode configurations. Several different detection approaches are described, including dual-channel systems for dual-electrode detection and electrochemistry coupled with fluorescence and chemiluminescence. The application of ME-EC to the determination of catecholamines, adenosine and its metabolites, and reactive nitrogen and oxygen species in microdialysis samples and cell lysates is also detailed. Lastly, approaches for coupling of ME-EC with microdialysis sampling to create separation-based sensors that can be used for near real-time monitoring of drug metabolism and neurotransmitters in freely roaming animals are provided.
引用
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页码：6101 / 6119
页数：18
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