An efficient multiplex genotyping approach for detecting the major worldwide human Y-chromosome haplogroups

被引：0
作者：
Mannis van Oven
Arwin Ralf
Manfred Kayser
机构：
[1] Erasmus MC University Medical Center Rotterdam,Department of Forensic Molecular Biology
来源：
International Journal of Legal Medicine | 2011年 / 125卷
关键词：
Y chromosome; Y-SNP; Haplogroup; Patrilineal ancestry; Bio-geographic ancestry; Multiplex SNaPshot;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The Y chromosome is paternally inherited and therefore serves as an evolutionary marker of patrilineal descent. Worldwide DNA variation within the non-recombining portion of the Y chromosome can be represented as a monophyletic phylogenetic tree in which the branches (haplogroups) are defined by at least one SNP. Previous human population genetics research has produced a wealth of knowledge about the worldwide distribution of Y-SNP haplogroups. Here, we apply previous and very recent knowledge on the Y-SNP phylogeny and Y-haplogroup distribution by introducing two multiplex genotyping assays that allow for the hierarchical detection of 28 Y-SNPs defining the major worldwide Y haplogroups. PCR amplicons were kept small to make the method sensitive and thereby applicable to DNA of limited amount and/or quality such as in forensic settings. These Y-SNP assays thus form a valuable tool for researchers in the fields of forensic genetics and genetic anthropology to infer a man's patrilineal bio-geographic ancestry from DNA.
引用
收藏
相关论文
共 87 条
[1]  
Altshuler DM(2010)Integrating common and rare genetic variation in diverse human populations Nature 467 52-58
[2]  
Gibbs RA(2007)SNPlexing the human Y-chromosome: a single-assay system for major haplogroup screening Electrophoresis 28 3201-3206
[3]  
Peltonen L(2005)Introduction of an single nucleodite polymorphism-based “Major Y-chromosome haplogroup typing kit” suitable for predicting the geographical origin of male lineages Electrophoresis 26 4411-4420
[4]  
Berniell-Lee G(2009)Y chromosome diversity, human expansion, drift, and cultural evolution Proc Natl Acad Sci USA 106 20174-20179
[5]  
Sandoval K(2011)A revised root for the human Y chromosomal phylogenetic tree: the origin of patrilineal diversity in Africa Am J Hum Genet 88 1-5
[6]  
Mendizabal I(2008)New binary polymorphisms reshape and increase resolution of the human Y chromosomal haplogroup tree Genome Res 18 830-838
[7]  
Bosch E(2010)Evaluating self-declared ancestry of U.S. Americans with autosomal, Y-chromosomal and mitochondrial DNA Hum Mutat 31 E1875-E1893
[8]  
Comas D(2011)Increased resolution of Y chromosome haplogroup T defines relationships among populations of the Near East, Europe, and Africa Hum Biol 83 39-53
[9]  
Brión M(2011)A major Y-chromosome haplogroup R1b Holocene era founder effect in Central and Western Europe Eur J Hum Genet 19 95-101
[10]  
Sanchez JJ(2006)Development of multiplex PCRs for evolutionary and forensic applications of 37 human Y chromosome SNPs Forensic Sci Int 157 23-35
123456789