Inhibition effects of some pesticides and heavy metals on carbonic anhydrase enzyme activity purified from horse mackerel (Trachurus trachurus) gill tissues

被引：0

作者：

Cuneyt Caglayan

Parham Taslimi

Cebrahil Türk

İlhami Gulcin

Fatih Mehmet Kandemir

Yeliz Demir

Şükrü Beydemir

机构：

[1] Bingol University,Department of Biochemistry, Faculty of Veterinary Medicine

[2] Bartin University,Department of Biotechnology, Faculty of Science

[3] Bingol University,Department of Fisheries, Genç Vocational School

[4] Atatürk University,Department of Chemistry, Faculty of Science

[5] Atatürk University,Department of Biochemistry, Faculty of Veterinary Medicine

[6] Ardahan University,Department of Pharmacy Services, Nihat Delibalta Göle Vocational High School

[7] Anadolu University,Department of Biochemistry, Faculty of Pharmacy

来源：

Environmental Science and Pollution Research | 2020年 / 27卷

关键词：

Carbonic anhydrase; Horse mackerel; Gill; Heavy metal; Pesticides;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The gill tissue is the main site of metabolic enzymes or compensation, with the kidney tissue playing a supporting role. At the gill tissue, carbonic anhydrase enzymes (CAs) catalyze the hydration of CO2 to HCO3− and H+ for production to the H2O. In this work, the CA enzyme was purified from horse mackerel (Trachurus trachurus) gill with a specific activity of 21,381.42 EU/mg, purification fold of 150.61, total activity of 2347.68 EU/mL, and a yield of 16.13% using sepharose 4B-L-tyrosine-sulfanilamide affinity gel chromatography. For recording the enzyme purity, gel electrophoresis was performed, and single band was seen. The molecular weight of this enzyme was found approximately 35 kDa. Also, the inhibitory effects of different pesticides such as thiram, clofentezine, propineb, deltamethrin, azoxystrobin, and thiophanate and heavy metal ions such as Fe2+, Cu2+, Co2+, Pb2+ Hg2+, and As3+ on horse mackerel gill tissue CA enzyme activities were investigated. Our results indicated that these pesticides and metal ions showed inhibitory effects at low nanomolar and millimolar concentrations for fish gill CA enzymes, respectively.

引用

页码：10607 / 10616

页数：9

共 245 条

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