Monitoring the biofertilizing potential and establishment of inoculated cyanobacteria in soil using physiological and molecular markers

被引：1
作者：
Radha Prasanna
Ranvijay Narayan Singh
Monica Joshi
Komal Madhan
Ram Krishna Pal
Lata Nain
机构：
[1] Division of Microbiology,
[2] Indian Agricultural Research Institute (IARI),undefined
[3] Division of Post Harvest Technology,undefined
[4] Indian Agricultural Research Institute (IARI),undefined
来源：
Journal of Applied Phycology | 2011年 / 23卷
关键词：
Amplification profiles; Cyanobacteria; Chlorophyll; Nitrogen fixation; Repeat sequences;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
This paper aims to develop methods for quantifying their establishment; using physiological activity (chlorophyll as a growth index and nitrogen-fixing potential as a measure of their biofertilizing capacity), along with evaluation based on DNA fingerprints generated using repeat sequences/palindromes. Time course studies were undertaken in liquid and soil microcosm experiments inoculated with a set of four rhizosphere cyanobacterial strains (BF1 Anabaena sp., BF2 Nostoc sp., BF3 Nostoc sp., BF4 Anabaena sp.). Observations revealed the synergistic effect of three-membered combinations (especially the i.e. BF1 + 2 + 3, 1 + 2 + 4, 1 + 3 + 4) in terms of enhancing chlorophyll and acetylene reducing activity. PCR-based amplification profiles (using short tandemly repetitive repeat (STRR) 1A, STRRmod, and HIPAT sequences) proved discriminative in monitoring the presence of the inoculated cyanobacteria in soil microcosm. Future work is in progress to assess the utility of the selected markers/primers in pot experiments, followed by field-level experiments with crop.
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页码：301 / 308
页数：7
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