One-step transformation of the dimorphic yeast Yarrowia lipolytica

被引：0
作者：
D.-C. Chen
J.-M. Beckerich
C. Gaillardin
机构：
[1] Laboratoire de Genétique Moléculaire et Cellulaire,
[2] Institut National de la Recherche Agromique-CNRS,undefined
[3] Centre de Biotechnologie Agro-Industrielle,undefined
[4] Institut National Agronomique Paris-Grignon,undefined
[5] F-78850,undefined
[6] Thiverval-Grignon,undefined
[7] Paris,undefined
[8] France Tel.: (+33 1) 30815443 Fax: (+33 1) 30815457,undefined
来源：
Applied Microbiology and Biotechnology | 1997年 / 48卷
关键词：
Ethylene; Agar; Ethylene Glycol; Heat Shock; Cell Density;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
An efficient one-step transformation method for the dimorphic yeast Yarrowia lipolytica is described. Using cells grown overnight on agar plates, the whole process is carried out within 1 h. The transformant clones could be recovered on selective plates as early as 36–48 h after plating. The efficiency was better than 105 transformants/μg replicative plasmid DNA. Effects of cell density, dithiothreitol, heat shock, poly(ethylene glycol) 4000 concentration and the wetness of selective plates were investigated.
引用
收藏
页码：232 / 235
页数：3
相关论文
共 50 条
[41]   A one-step process to produce high-crystallinity cellulose microfibrils from microwave irradiation of natural fiber waste [J].
Romero-Zuniga, G. Y. ;
Sanchez-Valdes, S. ;
Ceniceros-Reyes, M. A. ;
Sifuentes-Nieves, I. ;
Gallardo-Vega, C. A. ;
Solis-Rosales, S. G. ;
Gonzalez-Morones, P. ;
Hernandez-Hernandez, E. .
CELLULOSE, 2023, 30 (16) :10067-10082
[42]   One-step synthesis of nitrogen-doped carbon nanofibers from melamine over nickel alloy in a closed system [J].
Kenzhin, Roman M. ;
Bauman, Yuri I. ;
Volodin, Alexander M. ;
Mishakov, Ilya V. ;
Vedyagin, Aleksey A. .
CHEMICAL PHYSICS LETTERS, 2017, 685 :259-262
[43]   Electroless silver plating on Pb-based glass frits by a one-step activation method without stannum and palladium [J].
Huang, Bei ;
Gan, Weiping ;
Guo, Guiquan ;
Li, Yingfen ;
Lin, Tao ;
Liu, Xiaogang .
CERAMICS INTERNATIONAL, 2014, 40 (01) :393-397
[44]   CrOx-ZrO2-SiO2 Catalysts for Nonoxidative Propane Dehydrogenation, Prepared by Impregnation and One-Step Precipitation of the Components [J].
Golubina, E. V. ;
Kaplin, I. Yu. ;
Gorodnova, A. V. ;
Lokteva, E. S. ;
Isaikina, O. Ya. ;
Maslakov, K. I. .
RUSSIAN JOURNAL OF APPLIED CHEMISTRY, 2022, 95 (11) :1677-1692
[45]   One-step purification of xylanase from Melanocarpus albomyces and ethylene glycol as a novel soluble additive for enhancing its thermal stability [J].
Anshu Gupta ;
Ipsita Roy ;
S.K. Khare ;
V.S. Bisaria ;
M.N. Gupta .
Biotechnology Letters, 2002, 24 :2005-2009
[46]   Oxidation-resistant micron-sized Cu-Sn solid particles fabricated by a one-step and scalable method [J].
Liang, Yujia ;
Oh, Su Cheun ;
Wang, Xizheng ;
Glicksman, Howard ;
Liu, Dongxia ;
Ehrman, Sheryl .
RSC ADVANCES, 2017, 7 (38) :23468-23477
[47]   Viability of bovine blastocysts obtained after 7, 8 or 9 days of culture in vitro following vitrification and one-step rehydration [J].
Saha, S ;
Rajamahendran, R ;
Boediono, A ;
Sumantri, C ;
Suzuki, T .
THERIOGENOLOGY, 1996, 46 (02) :331-343
[48]   One-step purification of xylanase from Melanocarpus albomyces and ethylene glycol as a novel soluble additive for enhancing its thermal stability [J].
Gupta, A ;
Roy, I ;
Khare, SK ;
Bisaria, VS ;
Gupta, MN .
BIOTECHNOLOGY LETTERS, 2002, 24 (23) :2005-2009
[49]   One-step extraction and quantization of toxic alcohols and ethylene glycol in plasma by capillary gas chromatography (GC) with flame ionization detection (FID) [J].
Orton, Dennis J. ;
Boyd, Jessica M. ;
Affleck, Darlene ;
Duce, Donna ;
Walsh, Warren ;
Seiden-Long, Isolde .
CLINICAL BIOCHEMISTRY, 2016, 49 (1-2) :132-138
[50]   One-Step Synthesis of CuxOy/TiO2 Photocatalysts by Laser Pyrolysis for Selective Ethylene Production from Propionic Acid Degradation [J].
Karpiel, Juliette ;
Lonchambon, Pierre ;
Dappozze, Frederic ;
Florea, Ileana ;
Dragoe, Diana ;
Guillard, Chantal ;
Herlin-Boime, Nathalie .
NANOMATERIALS, 2023, 13 (05)
12345