One-step transformation of the dimorphic yeast Yarrowia lipolytica

被引：0
作者：
D.-C. Chen
J.-M. Beckerich
C. Gaillardin
机构：
[1] Laboratoire de Genétique Moléculaire et Cellulaire,
[2] Institut National de la Recherche Agromique-CNRS,undefined
[3] Centre de Biotechnologie Agro-Industrielle,undefined
[4] Institut National Agronomique Paris-Grignon,undefined
[5] F-78850,undefined
[6] Thiverval-Grignon,undefined
[7] Paris,undefined
[8] France Tel.: (+33 1) 30815443 Fax: (+33 1) 30815457,undefined
来源：
Applied Microbiology and Biotechnology | 1997年 / 48卷
关键词：
Ethylene; Agar; Ethylene Glycol; Heat Shock; Cell Density;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
An efficient one-step transformation method for the dimorphic yeast Yarrowia lipolytica is described. Using cells grown overnight on agar plates, the whole process is carried out within 1 h. The transformant clones could be recovered on selective plates as early as 36–48 h after plating. The efficiency was better than 105 transformants/μg replicative plasmid DNA. Effects of cell density, dithiothreitol, heat shock, poly(ethylene glycol) 4000 concentration and the wetness of selective plates were investigated.
引用
收藏
页码：232 / 235
页数：3
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