One-step transformation of the dimorphic yeast Yarrowia lipolytica

被引：0
|
作者：
D.-C. Chen
J.-M. Beckerich
C. Gaillardin
机构：
[1] Laboratoire de Genétique Moléculaire et Cellulaire,
[2] Institut National de la Recherche Agromique-CNRS,undefined
[3] Centre de Biotechnologie Agro-Industrielle,undefined
[4] Institut National Agronomique Paris-Grignon,undefined
[5] F-78850,undefined
[6] Thiverval-Grignon,undefined
[7] Paris,undefined
[8] France Tel.: (+33 1) 30815443 Fax: (+33 1) 30815457,undefined
来源：
Applied Microbiology and Biotechnology | 1997年 / 48卷
关键词：
Ethylene; Agar; Ethylene Glycol; Heat Shock; Cell Density;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
An efficient one-step transformation method for the dimorphic yeast Yarrowia lipolytica is described. Using cells grown overnight on agar plates, the whole process is carried out within 1 h. The transformant clones could be recovered on selective plates as early as 36–48 h after plating. The efficiency was better than 105 transformants/μg replicative plasmid DNA. Effects of cell density, dithiothreitol, heat shock, poly(ethylene glycol) 4000 concentration and the wetness of selective plates were investigated.
引用
收藏
页码：232 / 235
页数：3
相关论文
共 50 条
  • [1] One-step transformation of the dimorphic yeast Yarrowia lipolytica
    Chen, DC
    Beckerich, JM
    Gaillardin, C
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1997, 48 (02) : 232 - 235
  • [2] One-step integration of multiple genes into the oleaginous yeast Yarrowia lipolytica
    Gao, Shuliang
    Han, Linna
    Zhu, Li
    Ge, Mei
    Yang, Sheng
    Jiang, Yu
    Chen, Daijie
    BIOTECHNOLOGY LETTERS, 2014, 36 (12) : 2523 - 2528
  • [3] One-step integration of multiple genes into the oleaginous yeast Yarrowia lipolytica
    Shuliang Gao
    Linna Han
    Li Zhu
    Mei Ge
    Sheng Yang
    Yu Jiang
    Daijie Chen
    Biotechnology Letters, 2014, 36 : 2523 - 2528
  • [4] INTEGRATIVE TRANSFORMATION OF THE YEAST YARROWIA-LIPOLYTICA
    DAVIDOW, LS
    APOSTOLAKOS, D
    ODONNELL, MM
    PROCTOR, AR
    OGRYDZIAK, DM
    WING, RA
    STASKO, I
    DEZEEUW, JR
    CURRENT GENETICS, 1985, 10 (01) : 39 - 48
  • [5] Serum-induced hypha formation in the dimorphic yeast Yarrowia lipolytica
    Kim, J
    Cheon, SA
    Park, S
    Song, Y
    Kim, JY
    FEMS MICROBIOLOGY LETTERS, 2000, 190 (01) : 9 - 12
  • [6] Biochemical and genetic characterization of phosphofructokinase from the dimorphic yeast Yarrowia Lipolytica
    Martínez-Costa, OH
    Flores, CL
    Sánchez, V
    Gancedo, C
    Aragón, JJ
    FEBS JOURNAL, 2005, 272 : 14 - 14
  • [7] ONE-STEP TRANSFORMATION OF YEAST IN STATIONARY PHASE
    CHEN, DC
    YANG, BC
    KUO, TT
    CURRENT GENETICS, 1992, 21 (01) : 83 - 84
  • [8] High cell density culture of Yarrowia lipolytica using a one-step feeding process
    Kim, JW
    Park, TJ
    Ryu, DDY
    Kim, JY
    BIOTECHNOLOGY PROGRESS, 2000, 16 (04) : 657 - 660
  • [9] Roles of the three Ras proteins in the regulation of dimorphic transition in the yeast Yarrowia lipolytica
    Li, Min
    Li, Yun-Qing
    Zhao, Xiao-Feng
    Gao, Xiang-Dong
    FEMS YEAST RESEARCH, 2014, 14 (03) : 451 - 463
  • [10] A Rac homolog is required for induction of hyphal growth in the dimorphic yeast Yarrowia lipolytica
    Hurtado, CAR
    Beckerich, JM
    Gaillardin, C
    Rachubinski, RA
    JOURNAL OF BACTERIOLOGY, 2000, 182 (09) : 2376 - 2386
12345