Productive HIV-1 infection is enriched in CD4-CD8- double negative (DN) T cells at pleural sites of dual infection with HIV and Mycobacterium tuberculosis

被引：0
作者：
Qinglai Meng
David H. Canaday
David J. McDonald
Harriet Mayanja-Kizza
Joy Baseke
Zahra Toossi
机构：
[1] Case Western Reserve University,Division of Infectious Diseases, Department of Medicine, BRB 10W
[2] Veterans Affairs Medical Center,Department of Molecular Biology and Microbiology
[3] Case Western Reserve University,undefined
[4] Makerere University,undefined
[5] Joint Clinical Research Center,undefined
来源：
Archives of Virology | 2016年 / 161卷
关键词：
T cell; Tuberculosis; HIV-1; PFMC; Double-negative T cell;
D O I：
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中图分类号：
学科分类号：
摘要：
A higher human immunodeficiency virus 1 (HIV-1) viral load at pleural sites infected with Mycobacterium tuberculosis (MTB) than in peripheral blood has been documented. However, the cellular source of productive HIV infection in HIV-1/MTB-coinfected pleural fluid mononuclear cells (PFMCs) remains unclear. In this study, we observed significant quantities of HIV-1 p24+ lymphocytes in PFMCs, but not in peripheral blood mononuclear cells (PBMCs). HIV-1 p24+ lymphocytes were mostly enriched in DN T cells. Intracellular CD4 expression was detectable in HIV-1 p24+ DN T cells. HIV-1 p24+ DN T cells showed lower surface expression of human leukocyte antigen (HLA)-ABC and tetherin than did HIV-1 p24+ CD4 T cells. Upon in vitro infection of PFMC CD4 T cells from TB mono-infected subjects, Nef- and/or Vpu-deleted HIV mutants showed lower generation of HIV-1 p24+ DN T cells than the wild-type virus. These data indicate that productively HIV-1-infected DN T cells, generated through down-modulation of surface CD4, likely by HIV-1 Nef and Vpu, are the predominant source of HIV-1 at pleural sites of HIV/MTB coinfection.
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页码：181 / 187
页数：6
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