Productive HIV-1 infection is enriched in CD4-CD8- double negative (DN) T cells at pleural sites of dual infection with HIV and Mycobacterium tuberculosis

被引：0

作者：

Qinglai Meng

David H. Canaday

David J. McDonald

Harriet Mayanja-Kizza

Joy Baseke

Zahra Toossi

机构：

[1] Case Western Reserve University,Division of Infectious Diseases, Department of Medicine, BRB 10W

[2] Veterans Affairs Medical Center,Department of Molecular Biology and Microbiology

[3] Case Western Reserve University,undefined

[4] Makerere University,undefined

[5] Joint Clinical Research Center,undefined

来源：

Archives of Virology | 2016年 / 161卷

关键词：

T cell; Tuberculosis; HIV-1; PFMC; Double-negative T cell;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A higher human immunodeficiency virus 1 (HIV-1) viral load at pleural sites infected with Mycobacterium tuberculosis (MTB) than in peripheral blood has been documented. However, the cellular source of productive HIV infection in HIV-1/MTB-coinfected pleural fluid mononuclear cells (PFMCs) remains unclear. In this study, we observed significant quantities of HIV-1 p24+ lymphocytes in PFMCs, but not in peripheral blood mononuclear cells (PBMCs). HIV-1 p24+ lymphocytes were mostly enriched in DN T cells. Intracellular CD4 expression was detectable in HIV-1 p24+ DN T cells. HIV-1 p24+ DN T cells showed lower surface expression of human leukocyte antigen (HLA)-ABC and tetherin than did HIV-1 p24+ CD4 T cells. Upon in vitro infection of PFMC CD4 T cells from TB mono-infected subjects, Nef- and/or Vpu-deleted HIV mutants showed lower generation of HIV-1 p24+ DN T cells than the wild-type virus. These data indicate that productively HIV-1-infected DN T cells, generated through down-modulation of surface CD4, likely by HIV-1 Nef and Vpu, are the predominant source of HIV-1 at pleural sites of HIV/MTB coinfection.

引用

页码：181 / 187

页数：6

共 165 条

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