Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

被引：0

作者：

机构：

[1] UK Stem Cell Bank,Division of Cell Biology and Imaging

[2] National Institute for Biological Standards and Control,Department of Molecular Biology and Biotechnology

[3] South Mimms,Department for Laboratory Medicine

[4] Centre for Stem Cell Biology,Department of Biomedical Science

[5] The University of Sheffield,Department of Genetics

[6] Unit for Molecular Embryology,Department of Surgery

[7] Karolinska Institutet,Department of Pathology

[8] Rambam Medical Center,Department of Biology, and Department of Molecular Embryology

[9] Technion–Israel Institute of Technology,Department of Laboratory Medicine

[10] POB 9602,Department of Development and Genetics

[11] Centre for Stem Cell Biology,Department of Obstetrics and Gynecology

[12] The University of Sheffield,Department of Biochemical Engineering

[13] Geneservice Limited,The Goldyne Savad Institute of Gene Therapy & The Department of Obstetrics and Gynecology

[14] No.2 Cambridge Science Park,undefined

[15] Cancer Research UK Genotyping Facility,undefined

[16] St James's University Hospital,undefined

[17] Stem Cell Sciences Ltd.,undefined

[18] Building 75,undefined

[19] Monash University,undefined

[20] The Hebrew University of Jerusalem,undefined

[21] Institute of Life Sciences,undefined

[22] Edmond J. Safra Campus,undefined

[23] Givat Ram,undefined

[24] Matrix Technologies,undefined

[25] Thermo Fisher Scientific,undefined

[26] NIH Stem Cell Unit,undefined

[27] Bldg 35/2B-213,undefined

[28] MSC 3703,undefined

[29] National Institutes of Health,undefined

[30] Bioprocessing Technology Institute,undefined

[31] The Jackson Laboratory,undefined

[32] University of Cambridge,undefined

[33] Addenbrookes Hospital,undefined

[34] The University of Kansas,undefined

[35] School of Medicine,undefined

[36] Samuel Lunenfeld Research Institute,undefined

[37] Room 881,undefined

[38] Mount Sinai Hospital,undefined

[39] Faculty of Medicine,undefined

[40] Masaryk University,undefined

[41] Brno,undefined

[42] Czech Republic,undefined

[43] Institute of Experimental Medicine,undefined

[44] Academy of Sciences of the Czech Republic,undefined

[45] Cellartis AB,undefined

[46] Arvid Wallgrens Backe 20,undefined

[47] 413 46,undefined

[48] Monash Immunology and Stem Cell Laboratories (MISCL),undefined

[49] Level 3,undefined

[50] STRIP–Building 75,undefined

来源：

Nature Biotechnology | 2007年 / 25卷

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摘要：

The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue-nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.

引用

页码：803 / 816

页数：13

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