Harnessing Whole Genome Sequencing in Medical Mycology

被引：17
作者：
Cuomo C.A. [1 ]
机构：
[1] Infectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, MA
来源：
Current Fungal Infection Reports | 2017年 / 11卷 / 2期
基金：
美国国家卫生研究院;
关键词：
Fungal infections; Genome sequencing; Human fungal pathogens; Medical mycology; Review; Whole genome sequencing;
D O I：
10.1007/s12281-017-0276-7
中图分类号：
学科分类号：
摘要：
Purpose of Review: Comparative genome sequencing studies of human fungal pathogens enable identification of genes and variants associated with virulence and drug resistance. This review describes current approaches, resources, and advances in applying whole genome sequencing to study clinically important fungal pathogens. Recent Findings: Genomes for some important fungal pathogens were only recently assembled, revealing gene family expansions in many species and extreme gene loss in one obligate species. The scale and scope of species sequenced is rapidly expanding, leveraging technological advances to assemble and annotate genomes with higher precision. By using iteratively improved reference assemblies or those generated de novo for new species, recent studies have compared the sequence of isolates representing populations or clinical cohorts. Whole genome approaches provide the resolution necessary for comparison of closely related isolates, for example, in the analysis of outbreaks or sampled across time within a single host. Summary: Genomic analysis of fungal pathogens has enabled both basic research and diagnostic studies. The increased scale of sequencing can be applied across populations, and new metagenomic methods allow direct analysis of complex samples. © 2017, Springer Science+Business Media New York.
引用
收藏
页码：52 / 59
页数：7
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