New construction of an animal model for the orthotopic transplantation of an ovarian tumor

被引：0

作者：

Hui Zhang

Xinping Gao

Yongan Yang

Weiming Wang

Jin Liu

Yijuan Liang

Hongli Wu

Jinjin Qin

Kun Pan

Yifeng Wang

Junrong Shi

Youju Ma

机构：

[1] Affiliated Hospital Of HeBei University,Department of Obstetrics and Gynecology

[2] The Third Hospital Of Baoding/Baoding Cancer Hospital,undefined

[3] ZhuJiang Hospital of Southem Medical University,undefined

来源：

Journal of Ovarian Research | / 7卷

关键词：

Animal model; Orthotopic transplantation; Tumor; Ovarian cancer;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A new technique has successfully established the non-obese diabetic/severely combined immunodeficiency (NOD/SCID) mouse model of ovarian cancer. Under 4% chloral hydrate (0.1 mL/g dose) anesthesia, female mice were inoculated with tumor-cell suspension. The expression rate of OVCAR3 to CA125 was assessed using flow cytometry. The inoculated site was hand palpated and the signs and symptoms related to tumor growth were observed with the naked eye. The allophycocyanin (APC) indirectly labeled mouse-antihuman CA125 and fluorescein isothiocyanate (FITC)-labeled anti-mouse MHC Class I molecule (H-2Kd/H-2Dd) were observed using a confocal laser scanning microscope. The animal model of ovarian cancer constructed using this method can more directly reflect the characteristics of cancer cells. It provides reliable experimental results and presents a technical platform for the research of ovarian cancer stem cells.

引用

共 50 条

[21] Orthotopic ovarian tissue transplantation: Feasibility of pregnancy
Donnez, J
Dolmans, MM
Demylle, D
Martinez-Madrid, B
Van Langendonckt, A
Proceedings of the 13th World Congress on In Vitro Fertilization, Assisted Reproduction & Genetics, 2005, : 697 - 704
[22] A new experimental model of orthotopic lung transplantation: Padova experience
Nannini, N.
Lunardi, F.
Vadori, M.
Dedja, A.
Cozzi, E.
Rea, F.
Calabrese, F.
VIRCHOWS ARCHIV, 2012, 461 : S53 - S54
[23] Orthotopic aortic transplantation in mice: A new model of allograft arteriosclerosis
Dambrin, C
Calise, D
Pieraggi, MT
Thiers, JC
Thomsen, M
JOURNAL OF HEART AND LUNG TRANSPLANTATION, 1999, 18 (10): : 946 - 951
[24] Back muscle as a promising site for ovarian tissue transplantation, an animal model
Soleimani, R.
Van der Elst, J.
Heytens, E.
Van den Broecke, R.
Gerris, J.
Dhont, M.
Cuvelier, C.
De Sutter, P.
HUMAN REPRODUCTION, 2008, 23 (03) : 619 - 626
[25] Effects of sulforaphane on bladder cancer in vitro and in an orthotopic bladder tumor animal model.
Zhou, Jin-Rong
Singh, Ajita
Blackburn, George
CANCER RESEARCH, 2006, 66 (08)
[26] Livebirth after orthotopic transplantation of cryopreserved ovarian tissue
Donnez, J
Dolmans, MM
Demylle, D
Jadoul, P
Pirard, C
Squifflet, J
Martinez-Madrid, B
Van Langendonckt, A
Human Reproduction, 2005, : 298 - 298
[27] Laparoscopic autologous orthotopic and heterotopic ovarian transplantation in rabbits
Sortsis, A.
Pados, G.
Tarlatzis, B. K.
Saratsi, A.
Savas, I.
Skepastianos, P.
Tzioufa, V.
Kazakos, G.
Sdrani, V.
Bontis, I. N.
PROCEEDINGS OF THE 14TH ANNUAL CONGRESS OF THE EUROPEAN SOCIETY FOR GYNAECOLOGICAL ENDOSCOPY: ADVANCED MINIMAL INVASIVE SURGERY IN THE THEATRE IN THE OFFICE, 2005, : 103 - 108
[28] Imaging of Tumor-Associated Macrophages in a Transgenic Mouse Model of Orthotopic Ovarian Cancer
He, Huanhuan
Chiu, Alan C.
Kanada, Masamitsu
Schaar, Bruce T.
Krishnan, Venkatesh
Contag, Christopher H.
Dorigo, Oliver
MOLECULAR IMAGING AND BIOLOGY, 2017, 19 (05) : 694 - 702
[29] Livebirth after orthotopic transplantation of cryopreserved ovarian tissue
Donnez, J
Dolmans, MM
Demylle, D
Jadoul, P
Pirard, C
Squifflet, J
Martinez-Madrid, B
Van Langendonckt, A
LANCET, 2004, 364 (9443): : 1405 - 1410
[30] Imaging of Tumor-Associated Macrophages in a Transgenic Mouse Model of Orthotopic Ovarian Cancer
Huanhuan He
Alan C. Chiu
Masamitsu Kanada
Bruce T. Schaar
Venkatesh Krishnan
Christopher H. Contag
Oliver Dorigo
Molecular Imaging and Biology, 2017, 19 : 694 - 702

← 1 2 3 4 5 →