An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose

被引：0

作者：

Zhanchao Wang

Ming Chen

Yuquan Xu

Shuying Li

Wei Lu

Shuzhen Ping

Wei Zhang

Min Lin

机构：

[1] China Agricultural University,College of Biological Sciences

[2] Chinese Academy of Agricultural Science,Biotechnology Research Institute

来源：

Biotechnology Letters | 2008年 / 30卷

关键词：

Alcohol dehydrogenase; Ethanol; Pyruvate decarboxylase; Xylose;

D O I：

暂无

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学科分类号：

摘要：

An ethanol-tolerant mutant, ET1, was isolated by an enrichment method from Escherichia coli JM109. Strains JM109 and ET1 were transformed with expression vector pZY507bc containing Zymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes, resulting in an ethanol-sensitive recombinant strain JMbc and an ethanol-tolerant recombinant strain, ET1bc. Alcohol dehydrogenase and pyruvate decarboxylase activities were 24 and 32% lower, respectively, in JMbc than in ET1bc. ET1bc fermented 10% (w/v) xylose to give 39.4 g ethanol/l (77%, theoretical yield), a 1.3-fold increase compared with the ethanol-sensitive strain JMbc.

引用

页码：657 / 663

页数：6